Functional conservation of the grapevine candidate gene INNER NO OUTER for ovule development and seed formation  

作　　者：Valentina di Rienzo Zahra Imanifard Isabella Mascio Charles SGasser Debra JSkinner Ciro Leonardo Pierri Martina Marini Valentina Fanelli Wilma Sabetta Cinzia Montemurro Diana Bellin 

机构地区：[1]Department of Soil,Plant and Food Sciences,Section of Genetics and Breeding,University of Bari Aldo Moro,via Amendola 165/A,70125 Bari,Italy [2]Spin off Sinagri s.r.l.,University of Bari Aldo Moro,via Amendola 165/A,70125 Bari,Italy [3]Department of Biotechnology,University of Verona,Strada le Grazie 15,37134 Verona,Italy [4]Department of Molecular and Cellular Biology,University of California,Davis,1 Shields Ave.,Davis,CA 95616,USA [5]Department of Biosciences,Biotechnologies and Biopharmaceutics,Laboratory of Biochemistry Molecular and Structural Biology,University of Bari Aldo Moro,Via E.Orabona 4,70126 Bari,Italy [6]Spin off BROWSer S.r.l.(https://browser-bioinf.com/)c/o Department of Biosciences,Biotechnologies,Biopharmaceutics,University of Bari Aldo Moro,Via E.Orabona 4,70126 Bari,Italy [7]Institute of Biosciences and Bioresources of the National Research Council(IBBR-CNR),Via Amendola 165/A,70125 Bari,Italy [8]Institute for Sustainable Plant Protection-Support Unit Bari,National Research Council of Italy(CNR),Via Amendola 165/A,70125 Bari,Italy

出　　处：《Horticulture Research》2021年第1期279-291,共13页园艺研究（英文）

摘　　要：Seedlessness represents a highly appreciated trait in table grapes.Based on an interesting case of seedless fruit production described in the crop species Annona squamosa,we focused on the Vitis vinifera INNER NO OUTER(INO)gene as a candidate.This gene encodes a transcription factor belonging to the YABBY family involved in the determination of abaxial identity in several organs.In Arabidopsis thaliana,this gene was shown to be essential for the formation and asymmetric growth of the ovule outer integument and its mutation leads to a phenotypic defect of ovules and failure in seed formation.In this study,we identified in silico the V.vinifera orthologue and investigated its phylogenetic relationship to INO genes from other species and its expression in different organs in seeded and seedless varieties.Applying cross-species complementation,we have tested its functionality in the Arabidopsis ino-1 mutant.We show that the V.vinifera INO successfully rescues the ovule outer integument growth and seeds set and also partially complements the outer integument asymmetric growth in the Arabidopsis mutant,differently from orthologues from other species.These data demonstrate that VviINO retains similar activity and protein targets in grapevine as in Arabidopsis.Potential implications for grapevine breeding are discussed.

关 键 词：ARABIDOPSIS ORGANS outer 

分 类 号：S663.1[农业科学—果树学]