The H^(+)-pyrophosphatase IbVP1 regulates carbon flux to influence the starch metabolism and yield of sweet potato  

作　　者：Weijuan Fan Yandi Zhang Yinliang Wu Wenzhi Zhou Jun Yang Ling Yuan Peng Zhang Hongxia Wang 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [2]Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Plant Science Research Center,Chinese Academy of Sciences,Shanghai 201602,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Department of Plant and Soil Sciences and Kentucky Tobacco Research and Development Center,University of Kentucky,Lexington,KY 40546,USA

出　　处：《Horticulture Research》2021年第1期343-354,共12页园艺研究（英文）

基　　金：the National Key R&D Program of China(2018YFD1000700,2018YFD1000705,2019YFD1000701-2);the National Natural Science Foundation of China(31501356);the Special Funds for the Local Science and Technology Development of the Central Government(2020ZY0006);the Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops,Ministry of Agriculture and Rural Affairs,China(No.NYBSL201801);the National Key Technology Research and Development Program of China(2015BAD15B01);the Natural Science Foundation of Shanghai(18ZR1413300).

摘　　要：Storage roots of sweet potato are important sink organs for photoassimilates and energy,and carbohydrate metabolism in storage roots affects yield and starch production.Our previous study showed that sweet potato H+-pyrophosphatase(IbVP1)plays a vital role in mitigating iron deficiency and positively controls fibrous root growth.However,its roles in regulating starch production in storage roots have not been investigated.In this study,we found that IbVP1 overexpression in sweet potato improved the photosynthesis ability of and sucrose content in source leaves and increased both the starch content in and total yield of sink tissues.Using 13C-labeled sucrose feeding,we determined that IbVP1 overexpression promotes phloem loading and sucrose long-distance transport and enhances Pi-use efficiency.In sweet potato plants overexpressing IbVP1,the expression levels of starch biosynthesis pathway genes,especially AGPase and GBSSI,were upregulated,leading to changes in the structure,composition,and physicochemical properties of stored starch.Our study shows that the IbVP1 gene plays an important role in regulating starch metabolism in sweet potato.Application of the VP1 gene in genetic engineering of sweet potato cultivars may allow the improvement of starch production and yield under stress or nutrient-limited conditions.

关 键 词：CULTIVAR METABOLISM STARCH 

分 类 号：S532[农业科学—作物学]