LIWRKY39 is involved in thermotolerance by activating LIMBF1c and interacting with LICaM3 in lily(Lilium longiflorum)  

作　　者：Liping Ding Ze Wu Renda Teng Sujuan Xu Xing Cao Guozhen Yuan Dehua Zhang Nianjun Teng 

机构地区：[1]Key Laboratory of Landscaping Agriculture,Ministry of Agriculture and Rural Affairs,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China [2]Baguazhou Science and Technology Innovation Center of Modern Horticulture Industry,Nanjing 210043,China [3]College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China [4]State Key Laboratory of Microbial Technology,Shandong University,Qingdao 266237,China [5]College of Life Science,Zaozhuang University,Zaozhuang 277160,China

出　　处：《Horticulture Research》2021年第1期482-495,共14页园艺研究（英文）

基　　金：the National Key R&D Program of China(Grant No.2019YFD1000400);the National Natural Science Foundation of China(31902055);the High Level Talent Project of the Top Six Talents in Jiangsu,China(NY-077);the Natural Science Foundation of Jiangsu Province,China(BK20190532);the Fundamental Research Funds for the Central Universities(KJQN202032)。

摘　　要：WRKY transcription factors(TFs)are of great importance in plant responses to different abiotic stresses.However,research on their roles in the regulation of thermotolerance remains limited.Here,we investigated the function of LlWRKY39 in the thermotolerance of lily(Lilium longiflorum‘white heaven’).According to multiple alignment analyses,LIWRKY39 is in the WRKY IId subclass and contains a potential calmodulin(CaM)-binding domain.Further analysis has shown that LlCaM3 interacts with LlWRKY39 by binding to its CaM-binding domain,and this interaction depends on Ca^(2).LIWRKY39 was induced by heat stress(HS),and the LIWRKY39-GFP fusion protein was detected in the nucleus.The thermotolerance of lily and Arabidopsis was increased with the ectopic overexpression of LlWRKY39.The expression of heat-related genes AtHSFA1,AtHSFA2,AtMBF1c,AtGolS1,AtDREB2A,AtWRKY39,and AtHSP101 was significantly elevated in transgenic Arabidopsis lines,which might have promoted an increase in thermotolerance.Then,the promoter of LlMBF1c was isolated from lily,and LlWRKY39 was found to bind to the conserved W-box element in its promoter to activate its activity,suggesting that LlWRKY39 is an upstream regulator of LlMBF1c.In addition,a dual-luciferase reporter assay showed that via protein interaction,LlCaM3 negatively affected LlWRKY39 in the transcriptional activation of LlMBF1c,which might be an important feedback regulation pathway to balance the LlWRKY39-mediated heat stress response(HSR).Collectively,these results imply that LlWRKY39 might participate in the HSR as an important regulator through Ca^(2+)-CaM and multiprotein bridging factor pathways.

关 键 词：ARABIDOPSIS alignment tolerance 

分 类 号：S682.29[农业科学—观赏园艺]