The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids  

作　　者：Shanshan Dong Min Liu Yang Liu Fei Chen Ting Yang Lu Chen Xingtan Zhang Xing Guo Dongming Fang Linzhou Li Tian Deng Zhangxiu Yao Xiaoan Lang Yiqing Gong Ernest Wu Yaling Wang Yamei Shen Xun Gong Huan Liu Shouzhou Zhang 

机构地区：[1]Laboratory of Southern Subtropical Plant Diversity,Fairy Lake Botanical Garden,Shenzhen&Chinese Academy of Sciences,Shenzhen 518004,China [2]State Key Laboratory of Agricultural Genomics,BGI-Shenzhen,Shenzhen 518083,China [3]Nanjing Forestry University,Nanjing 210037,China [4]Fujian Agriculture and Forestry University,Fuzhou 350000,China [5]University of British Columbia,Vancouver BC,Canada [6]Xi’an Botanical Garden,Xi’an 710061,China [7]Zhejiang Agriculture and Forestry University,Hangzhou 311300,China [8]Kunming Botanical Garden,Chinese Academy of Sciences,Kunming 650201,China [9]Department of Biology,University of Copenhagen,DK-2100 Copenhagen,Denmark

出　　处：《Horticulture Research》2021年第1期552-564,共13页园艺研究（英文）

基　　金：the National Key R&D Program of China(No.2019YFC1711000);the National Natural Science Foundation(No.31600171);the Shenzhen Urban Management Bureau Fund(No.201520);the Shenzhen Municipal Government of China(No.JCYJ20170817145512467)。

摘　　要：Magnolia biondii Pamp.(Magnoliaceae,magnoliids)is a phylogenetically,economically,and medicinally important ornamental tree species widely grown and cultivated in the north-temperate regions of China.Determining the genome sequence of M.biondii would help resolve the phylogenetic uncertainty of magnoliids and improve the understanding of individual trait evolution within the Magnolia genus.We assembled a chromosome-level reference genome of M.biondii using~67,~175,and~154Gb of raw DNA sequences generated via Pacific Biosciences single-molecule real-time sequencing,10X Genomics Chromium,and Hi-C scaffolding strategies,respectively.The final genome assembly was~2.22Gb,with a contig N50 value of 269.11 kb and a BUSCO complete gene percentage of 91.90%.Approximately 89.17%of the genome was organized into 19 chromosomes,resulting in a scaffold N50 of 92.86Mb.The genome contained 47,547 protein-coding genes,accounting for 23.47%of the genome length,whereas 66.48%of the genome length consisted of repetitive elements.We confirmed a WGD event that occurred very close to the time of the split between the Magnoliales and Laurales.Functional enrichment of the Magnolia-specific and expanded gene families highlighted genes involved in the biosynthesis of secondary metabolites,plant–pathogen interactions,and responses to stimuli,which may improve the ecological fitness and biological adaptability of the lineage.Phylogenomic analyses revealed a sister relationship of magnoliids and Chloranthaceae,which are sister to a clade comprising monocots and eudicots.The genome sequence of M.biondii could lead to trait improvement,germplasm conservation,and evolutionary studies on the rapid radiation of early angiosperms.

关 键 词：EVOLUTION SISTER RESOLVE 

分 类 号：Q94[生物学—植物学]