Building a cluster of NLR genes conferring resistance to pests and pathogens: the story of the Vat gene cluster in cucurbits  

作　　者：Veronique Chovelon Rafael Feriche-Linares Guillaume Barreau Joel Chadoeuf Caroline Callot Veronique Gautier Marie-Christine Le Paslier Aurelie Berad Patricia Faivre-Rampant Jacques Lagnel Nathalie Boissot 

机构地区：[1]INRAE,GAFL,84143 Montfavet,France [2]INRAE,CNRGV,31326 Castanet-Tolosan,France [3]INRAE,GDEC-Gentyane Plateform,63000 Clermont-Ferrand,France [4]Universite Paris-Saclay,INRAE,EPGV,91000 Evry-Courcouronnes,France

出　　处：《Horticulture Research》2021年第1期899-910,共12页园艺研究（英文）

基　　金：the French Ministere de l’agriculture et de l’alimentation(Vat&Co project-CASDAR−2017-2021)。

摘　　要：Most molecularly characterized plant resistance genes(R genes)belong to the nucleotide-binding-site-leucine-rich-repeat(NLR)receptor family and are prone to duplication and transposition with high sequence diversity.In this family,the Vat gene in melon is one of the few R genes known for conferring resistance to insect,i.e.,Aphis gossypii,but it has been misassembled and/or mispredicted in the whole genomes of Cucurbits.We examined 14 genomic regions(about 400 kb)derived from long-read assemblies spanning Vat-related genes in Cucumis melo,Cucumis sativus,Citrullus lanatus,Benincasa hispida,Cucurbita argyrosperma,and Momordica charantia.We built the phylogeny of those genes.Investigating the paleohistory of the Vat gene cluster,we revealed a step by step process beginning from a common ancestry in cucurbits older than 50 my.We highlighted Vat exclusively in the Cucumis genera,which diverged about 20 my ago.We then focused on melon,evaluating a minimum duplication rate of Vat in 80 wild and cultivated melon lines using generalist primers;our results suggested that duplication started before melon domestication.The phylogeny of 44 Vat-CDS obtained from 21 melon lines revealed gain and loss of leucine-rich-repeat domains along diversification.Altogether,we revealed the high putative recognition scale offered in melon based on a combination of SNPs,number of leucine-rich-repeat domains within each homolog and number of homologs within each cluster that might jointly confer resistance to a large pest and pathogen spectrum.Based on our findings,we propose possible avenues for breeding programs.

关 键 词：NLR CLUSTER resistance 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]