De novo assembly of a new Olea europaea genome accession using nano pore seque ncing  

作　　者：Guodong Rao Jianguo Zhang Xiaoxia Liu Chunfu Lin Huaigen Xin Li Xue Chenhe Wang 

机构地区：[1]State Key Laboratory of Tree Genetics and Breed ing,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China [2]Collaborative Innovation Center of Susta in able Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China [3]Key Laboratory of Tree Breeding and Cultivation,National Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China [4]MIANNING Yuansheng Agricultural Science and Technology Co.,Ltd.,Liangshan Yi Autonomous Prefecture Mianning County,Sichuan 615600,China [5]Biomarker Technologies Corporation,Beijing 101300,China

出　　处：《Horticulture Research》2021年第1期1179-1190,共12页园艺研究（英文）

基　　金：The National Key R&D Program of China(2018YFD1000603-3);Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry(CAFYBB2018QB001)。

摘　　要：Olive(Olea europaea L.)is internationally renowned for its high-end product,extra virgin olive oil.An incomplete genome of O.europaea was previously obtained using shotgun sequencing in 2016.To further explore the genetic and breeding utilization of olive,an updated draft genome of olive was obtained using Oxford Nanopore third-generation sequencing and Hi-C technology.Seven different assembly strategies were used to assemble the fi nal genome of 1.30 Gb,with contig and scaffold N50 sizes of4.67 Mb and 42.60 Mb,respectively.This greatly increased the quality of the olive genome.We assembled 1.1 Gb of sequences of the total olive genome to 23 pseudochromosomes by Hi-C,and 53,518 protein-coding genes were predicted in the current assembly.Comparative genomics analyses,including gene family expansion and contraction,whole-genome replication,phylogenetic analysis,and positive selection,were performed.Based on the obtained high-quality olive genome,a total of nine gene families with 202 genes were identi fi ed in the oleuropein biosynthesis pathway,which is twice the number ofgenes identi fi ed from the previous data.This new accession of the olive genome is of suf fi cient quality for genome-wide studies on gene function in olive and has provided a foundation for the molecular breeding of olive species.

关 键 词：BREEDING OLEA ASSEMBLY 

分 类 号：Q943.2[生物学—植物学]