CmRCD1 represses flowering by directly interacting with CmBBX8 in summer chrysanthemum  

作　　者：Lijun Wang Hua Cheng Qi Wang Chaona Si Yiman Yang Yao Yu Lijie Zhou Lian Ding Aiping Song Dongqing Xu Sumei Chen Weimin Fang Fadi Chen Jiafu Jiang 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Key Laboratory of Landscaping,Ministry of Agriculture and Rural Affairs,Key Laboratory of Biology of Ornamental Plants in East China,National Forestry and Grassland Administration,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China [2]State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China [3]Present address:College of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056038,China

出　　处：《Horticulture Research》2021年第1期1191-1199,共9页园艺研究（英文）

基　　金：the National Natural Science Foundation of China(31930100,31872146);a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

摘　　要：The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum.However,the detailed regulatory mechanism of CmBBX8-mediated flowering remains elusive.In this study,we revealed that RADICAL-INDUCED CELL DEATH 1(CmRCD1)physically associated with CmBBX8 through bimolecular fluorescence complementation(BiFC),pulldown and Coimmunoprecipitation(CoIP)assays.Furthermore,the RCD1-SRO1-TAF4(RST)domain of CmRCD1 and the B-box of CmBBX8 mediated their interaction.In addition,Luciferase(LUC)assays and electrophoretic mobility shift assay(EMSAs)showed that CmRCD1 repressed the transcriptional activity of CmBBX8 and interfered with its binding to the CmFTL1 promoter,thereby leading to delayed flowering in the summer chrysanthemum‘Yuuka’.These results provide insight into the molecular framework of CmRCD1-CmBBX8-mediated flowering in chrysanthemum.

关 键 词：interaction. thereby FLOWERING 

分 类 号：S682.11[农业科学—观赏园艺]