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作 者:Fengchao Cui Geli Taier Manli Li Xiaoxia Dai Nan Hang Xunzhong Zhang Xiangfeng Wang Kehua Wang
机构地区:[1]Department of Turfgrass Science and Engineering,College of Grassland Science and Technology,China Agricultural University,Beijing 100193,China [2]Department of Breeding and Seed Science,College of Grassland Science and Technology,China Agricultural University,Beijing 100193,China [3]School of Plant and Environmental Sciences,Virginia Polytechnic Institute and State University,Blacksburg,VA 24061,USA [4]National Maize Improvement Center,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100913,China
出 处:《Horticulture Research》2021年第1期1213-1228,共16页园艺研究(英文)
基 金:the National Natural Science Foundation of China(32071887,31472140);the Beijing Municipal Natural Science Foundation(6182025)。
摘 要:Cynodon species can be used for multiple purposes and have high economic and ecological significance.However,the genetic basis of the favorable agronomic traits of Cynodon species is poorly understood,partially due to the limited availability of genomic resources.In this study,we report a chromosome-scale genome assembly of a diploid Cynodon species,C.transvaalensis,obtained by combining Illumina and Nanopore sequencing,BioNano,and Hi-C.The assembly contains 282 scaffolds(~423.42 Mb,N50=5.37 Mb),which cover~93.2%of the estimated genome of C.transvaalensis(~454.4Mb).Furthermore,90.48%of the scaffolds(~383.08 Mb)were anchored to nine pseudomolecules,of which the largest was 60.78Mb in length.Evolutionary analysis along with transcriptome comparison provided a preliminary genomic basis for the adaptation of this species to tropical and/or subtropical climates,typically with dry summers.The genomic resources generated in this study will not only facilitate evolutionary studies of the Chloridoideae subfamily,in particular,the Cynodonteae tribe,but also facilitate functional genomic research and genetic breeding in Cynodon species for new leading turfgrass cultivars in the future.
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