Comparative transcriptome analyses shed light on carotenoid production and plastid development in melon fruit  

作　　者：Noam Chayut Hui Yuan Yuval Saar Yi Zheng Tianhu Sun Xuesong Zhou Anna Hermanns Elad Oren Adi Faigenboim Maixia Hui Zhangjun Fei Michael Mazourek Joseph Burger Yaakov Tadmor Li Li 

机构地区：[1]Department of Vegetable Research,ARO,Newe Ya’ar Research Center,P.O.Box 1021,Ramat Yishay 30095,Israel [2]Robert W.Holley Center for Agriculture and Health,USDA-Agricultural Research Service,Cornell University,Ithaca,NY 14853,USA [3]Plant Breeding and Genetics Section,School of Integrative Plant Science,Cornell University,Ithaca,NY 14853,USA [4]Boyce Thompson Institute,Ithaca,NY 14853,USA [5]State Key Laboratory of Crop Genetics&Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China [6]Present address:John Innes Centre,Norwich Research Park,Norwich,UK

出　　处：《Horticulture Research》2021年第1期1229-1241,共13页园艺研究（英文）

基　　金：the United States-Israel Binational Agricultural Research and Development Fund(grant no.US-4918-16CR);the Agriculture and Food Research Initiative competitive award(grant no.2019-67013-29162)from the USDA National Institute of Food and Agriculture,and the USDA-ARS fund.

摘　　要：Carotenoids,such asβ-carotene,accumulate in chromoplasts of various fleshy fruits,awarding them with colors,aromas,and nutrients.The Orange(CmOr)gene controlsβ-carotene accumulation in melon fruit by posttranslationally enhancing carotenogenesis and repressingβ-carotene turnover in chromoplasts.Carotenoid isomerase(CRTISO)isomerizes yellow prolycopene into red lycopene,a prerequisite for further metabolism intoβ-carotene.We comparatively analyzed the developing fruit transcriptomes of orange-colored melon and its two isogenic EMS-induced mutants,low-β(Cmor)and yofi(Cmcrtiso).The Cmor mutation in low-βcaused a major transcriptomic change in the mature fruit.In contrast,the Cmcrtiso mutation in yofi significantly changed the transcriptome only in early fruit developmental stages.These findings indicate that melon fruit transcriptome is primarily altered by changes in carotenoid metabolic flux and plastid conversion,but minimally by carotenoid composition in the ripe fruit.Clustering of the differentially expressed genes into functional groups revealed an association between fruit carotenoid metabolic flux with the maintenance of the photosynthetic apparatus in fruit chloroplasts.Moreover,large numbers of thylakoid localized photosynthetic genes were differentially expressed in low-β.CmOR family proteins were found to physically interact with light-harvesting chlorophyll a–b binding proteins,suggesting a new role of CmOR for chloroplast maintenance in melon fruit.This study brings more insights into the cellular and metabolic processes associated with fruit carotenoid accumulation in melon fruit and reveals a new maintenance mechanism of the photosynthetic apparatus for plastid development.

关 键 词：apparatus ORANGE METABOLISM 

分 类 号：S631.2[农业科学—蔬菜学]