The domestication of Cucurbita argyrosperma as revealed by the genome of its wild relative  

作　　者：Josue Barrera-Redondo Guillermo Sanchez-de la Vega Jonas A.Aguirre-Liguori Gabriela Castellanos-Morales Yocelyn T.Gutierrez-Guerrero Xitlali Aguirre-Dugua Erika Aguirre-Planter Maud I.Tenaillon Rafael Lira-Saade Luis E.Eguiarte 

机构地区：[1]Departamento de Ecologia Evolutiva,Instituto de Ecologia,Universidad Nacional Autonoma de Mexico,Circuito Exterior s/n Anexo al Jardin Botanico,04510 Ciudad de Mexico,Mexico [2]Department of Ecology and Evolutionary Biology,University of California,Irvine,CA 92697,USA [3]Departamento de Conservacion de la Biodiversidad,El Colegio de la Frontera Sur,Villahermosa,Carretera Villahermosa‐Reforma km 15.5 Rancheria El Guineo 2ªseccion,86280 Villahermosa,Tabasco,Mexico [4]Genetique Quantitative et Evolution-Le Moulon,Universite Paris-Saclay,Institut National de Recherche pour l’Agriculture,l’Alimentation et l’Environnement,Centre National de la Recherche Scientifique,AgroParisTech,Gif-sur-Yvette 91190,France [5]UBIPRO,Facultad de Estudios Superiores Iztacala,Universidad Nacional Autonoma de Mexico,Av.de los Barrios#1,Col.Los Reyes Iztacala,Tlalnepantla,Edo.de Mex 54090,Mexico

出　　处：《Horticulture Research》2021年第1期1287-1300,共14页园艺研究（英文）

摘　　要：Despite their economic importance and well-characterized domestication syndrome,the genomic impact of domestication and the identification of variants underlying the domestication traits in Cucurbita species(pumpkins and squashes)is currently lacking.Cucurbita argyrosperma,also known as cushaw pumpkin or silver-seed gourd,is a Mexican crop consumed primarily for its seeds rather than fruit flesh.This makes it a good model to study Cucurbita domestication,as seeds were an essential component of early Mesoamerican diet and likely the first targets of human-guided selection in pumpkins and squashes.We obtained population-level data using tunable Genotype by Sequencing libraries for 192 individuals of the wild and domesticated subspecies of C.argyrosperma across Mexico.We also assembled the first high-quality wild Cucurbita genome.Comparative genomic analyses revealed several structural variants and presence/absence of genes related to domestication.Our results indicate a monophyletic origin of this domesticated crop in the lowlands of Jalisco.We found evidence of gene flow between the domesticated and wild subspecies,which likely alleviated the effects of the domestication bottleneck.We uncovered candidate domestication genes that are involved in the regulation of growth hormones,plant defense mechanisms,seed development,and germination.The presence of shared selected alleles with the closely related species Cucurbita moschata suggests domestication-related introgression between both taxa.

关 键 词：CUCURBITA LIKELY pump 

分 类 号：S642.1[农业科学—蔬菜学]