Genetic analysis of the “head top shape” quality trait of Chinese cabbage and its association with rosette leaf variation  

作　　者：Xiaoxue Sun Ying Gao Yin Lu Xiaomeng Zhang Shuangxia Luo Xing Li Mengyang Liu Daling Feng Aixia Gu Xueping Chen Shuxin Xuan Yanhua Wang Shuxing Shen Guusje Bonnema Jianjun Zhao 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation,Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei,Collaborative Innovation Center of Vegetable Industry in Hebei,College of Horticulture,Hebei Agricultural University,071000 Baoding,China [2]Plant Breeding,Wageningen University and Research,Wageningen,The Netherlands

出　　处：《Horticulture Research》2021年第1期1374-1385,共12页园艺研究（英文）

基　　金：the National Natural Science Foundation of China(Grant Nos.32002054,31930098,and 31801857);he National Key R&D of China(Grant No.2016YFD0100204-17);the Science and Technology Support Program of Hebei(Grant No.360108);the Natural Science Foundation of Hebei(Grant No.C2020204122);the International Cooperation Project in the Science and Technology Support Program of Hebei(Grant Nos.2019YX023A and 17396315D)。

摘　　要：The agricultural and consumer quality of Chinese cabbage is determined by its shape.The shape is defined by the folding of the heading leaves,which defines the head top shape(HTS).The overlapping HTS,in which the heading leaves curve inward and overlap at the top,is the shape preferred by consumers.To understand the genetic regulation of HTS,we generated a large segregating F 2 population from a cross between pak choi and Chinese cabbage,with phenotypes ranging from nonheading to heading with either outward curving or inward curving overlapping heading leaves.HTS was correlated with plant height,outer/rosette leaf length,and petiole length.A high-density genetic map was constructed.Quantitative trait locus(QTL)analysis resulted in the identification of 22 QTLs for leafy head-related traits,which included five HTS QTLs.Bulked segregant analysis(BSA)was used to confirm HTS QTLs and identify candidate genes based on informative single-nucleotide polymorphisms.Interestingly,the HTS QTLs colocalized with QTLs for plant height,outer/rosette leaf,and petiole length,consistent with the observed phenotypic correlations.Combined QTL analysis and BSA laid a foundation for molecular marker-assisted breeding of Chinese cabbage HTS and directions for further research on the genetic regulation of this trait.

关 键 词：HEADING TRAIT BREEDING 

分 类 号：S634.1[农业科学—蔬菜学]