Interaction of gibberellin and other hormones in almond anthers: phenotypic and physiological changes and transcriptomic reprogramming  

作　　者：Peng Li Jia Tian Changkui Guo Shuping Luo Jiang Li 

机构地区：[1]College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi 830052,China [2]Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100,China [3]School of Agriculture and Food Science,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China

出　　处：《Horticulture Research》2021年第1期1415-1430,共16页园艺研究（英文）

基　　金：the National Natural Science Foundation of China(31660562 and 31770352).

摘　　要：Low temperature causes anther dysfunction,severe pollen sterility and,ultimately,major yield losses in crop plants.Previous studies have shown that the gibberellic acid(GA)metabolic pathway plays an important role in this process by regulating tapetum function and pollen development.However,the interaction mechanism of GA with other hormones mediating anther development is still unclear.Herein,we collected and analyzed almond(Amygdalus communis L.)anthers at the meiosis,tetrad,1-nucleus,and mature 2-nucleus stages.The growth rate per 1000 anthers exhibited a significant positive correlation with the total bioactive GA compound content,and the levels of all bioactive GA compounds were highest in the 1-nucleus pollen stage.GA 3 treatment experiments indicated that exogenous GA_(3) increased the levels of indole-3-acetic acid(IAA),trans-zeatin(tZ),and jasmonic acid(JA)and decreased the levels of salicylic acid(SA)and abscisic acid(ABA);moreover,GA_(3) improved pollen viability and quantities under cold conditions,whereas PP 333(paclobutrazol,an inhibitor of GA biosynthesis)was antagonistic with GA_(3) in controlling anther development.RNA-seq and qRT-PCR results showed that GA played an important role in anther development by regulating the expression of other phytohormone pathway genes,dehydration-responsive element-binding/C-repeat binding factor(DREB1/CBF)-mediated signaling genes,and anther development pathway genes.Our results reveal the novel finding that GA interacts with other hormones to balance anther development under normal-and low-temperature conditions in almond.

关 键 词：programming quantities finding 

分 类 号：S662.2[农业科学—果树学]