CRISPR/Cas9-mediated targeted mutation reveals a role for AN4 rather than DPL in regulating venation formation in the corolla tube of Petunia hybrida  

作　　者：Bin Zhang Xiaojing Xu Renwei Huang Sha Yang Mingyang Li Yulong Guo 

机构地区：[1]Chongqing Engineering Research Center for Floriculture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,400716 Chongqing,China [2]College of Agriculture,Guizhou University,550025 Guiyang,Guizhou,China [3]Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources,College of Chemistry and Life Sciences,Chengdu Normal University,611130 Chengdu,China

出　　处：《Horticulture Research》2021年第1期1798-1806,共9页园艺研究（英文）

基　　金：the National Natural Science of China(31272199);Fundamental Research Funds for the Central Universities(XDJK2020D038).

摘　　要：Venation is a common anthocyanin pattern displayed in flowers that confers important ornamental traits to plants.An anthocyanin-related R2R3-MYB transcription factor,DPL,has been proposed to regulate corolla tube venation in petunia plants.Here,however,we provide evidence redefining the role of DPL in petunia.A CRISPR/Cas9-mediated mutation of DPL resulted in the absence of the vein-associated anthocyanin pattern above the abaxial surface of the flower bud,but not corolla tube venation,thus indicating that DPL did not regulate the formation of corolla tube venation.Alternately,quantitative real-time PCR analysis demonstrated that the spatiotemporal expression pattern of another R2R3-MYB gene,AN4,coincided with the formation of corolla tube venation in petunia.Furthermore,overexpression of AN4 promoted anthocyanin accumulation by increasing the expression of anthocyanin biosynthesis genes.CRISPR/Cas9-mediated mutation of AN4 led to an absence of corolla tube venation,suggesting that this gene in fact determines this key plant trait.Taken together,the results presented here redefine the prime regulator of corolla tube venation,paving the way for further studies on the molecular mechanisms underlying the various venation patterns in petunia.

关 键 词：CRISPR/Cas9 pattern FLOWERS 

分 类 号：S68[农业科学—观赏园艺]