The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii  

作　　者：Dan Hou Ling Li Tengfei Ma Jialong Pei Zhongyu Zhao Mengzhu Lu Aimin Wu Xinchun Lin 

机构地区：[1]State Key Laboratory of Subt ropical Silviculture,Zhejiang A&F University,Lin'an,311300 Han gzhou,China [2]State Key Laboratory for Conser vati on and Utilization of Subtropical Agro-bioresources,South China Agricultural University,510642 Guangzhou,China [3]Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,College of Forestry and Landscape Architecture,South China Agricultural University,510642 Guangzhou,China

出　　处：《Horticulture Research》2021年第1期1864-1876,共13页园艺研究（英文）

基　　金：supported by grants from the Natural Science Foundation of Zhejiang Province(LZ20C160002);the National Natural Science Foundation of China(31971735);the State Key Lab oratory of Subtropical Silviculture(ZY20180203).

摘　　要：Bamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value.Unique among traditional flowering plants,many bamboo plants undergo extensive synchronized flowering followed by large-scale death,seriously affecting the productivity and application of bamboo forests.To date,the molecular mechanism of bamboo flowering characteristics has remained unknown.In this study,a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1(SOC1)-like gene,BoMADS50,was identified from Bambusa oldhamii.BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B.oldhamii flowering and overexpression of BoMADS50 caused early fl owering in transgenic rice.Moreover,BoMADS50 could interact with APETALA1/FRUITFULL(AP1/FUL)-like proteins(BoMADS 14-1/2,BoMADS15-1/2)in vivo,and the expression of BoMADS50 was signi ficantly promoted by BoMADS14-1,further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B.oldhamii flowering.We also identi fi ed four additional transcripts of BoMADS50(BoMADS50-1/2/3/4)with different nucleotide variations.Although the protein-CDS were polymorphic,they had flowering activation functions similar to those of BoMADS50.Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE(SVP)-like gene BoSVP,but only BoMADS50-1 can positively induce their transcription.Therefore,nucleotide variations likely endow BoMADS50-1 with strong regulatory activity.Thus,BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B.oldhamii.Moreover,the functional conservatism and speci ficity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B.oldhamii.

关 键 词：FLOWERING TRANSIENT ACTIVATION 

分 类 号：Q943.2[生物学—植物学]