In tegrative iTRAQ-based proteomic and transcriptomic analysis reveals the accumulation patter ns of key metabolites associated with oil quality during seed ripening of Camellia oleifera  

作　　者：Zhouchen Ye Jing Yu Wuping Yan Junfeng Zhang Dongmei Yang Guanglong Yao Zijin Liu Yougen Wu Xilin Hou 

机构地区：[1]College of Horticulture,Hainan University,Haikou,China [2]State Key Laboratory of Crop Genetics&Germplasm Enhancement,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China),Ministry of Agriculture and Rural Affairs of the P.R.China,Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops,Ministry of Education of the P.R.China,Institute of Plasma Engineering,Nanjing,China

出　　处：《Horticulture Research》2021年第1期1997-2022,共26页园艺研究（英文）

基　　金：This study was supp or ted by the Funding for the Demon stration Fun ds for the Pr omoti on of Forestry Science and Technology from the Central Government([2020]TG 02);the Hainan Province Innovative Scientific Research Projects of Postg raduates(No.Hyb2019-01)。

摘　　要：Camellia oleifera(C.oleifera)is one of the four major woody oil-bearing crops in the world and has relatively high ecological,economic,and medicinal value.Its seeds undergo a series of complex physiological and biochemical changes during ripening,which is mainly manifested as the accumulation and transformation of certain metabolites closely related to oil quality,especially flavonoids and fatty acids.To obtain new insights into the underlying molecular mechanisms,a parallel analysis of the transcriptome and proteome profiles of C.oleifera seeds at different maturity levels was conducted using RNA sequencing(RNA-seq)and isobaric tags for relative and absolute quantification(iTRAQ)complemented with gas chromatography-mass spectrometry(GC-MS)data.A total of 16,530 transcripts and 1228 proteins were recognized with significant differential abundances in pairwise comparisons of samples at various developmental stages.Among these,317 were coexpressed with a poor correlation,and most were involved in metabolic processes,including fatty acid metabolism,a-linolenic acid metabolism,and glutathione metabolism.In addition,the content of total flavonoids decreased gradually with seed maturity,and the levels of fatty acids generally peaked at the fat accumulation stage;these results basically agreed with the regulation patterns of genes or proteins in the corresponding pathways.The expression levels of proteins annotated as upstream candidates of phenylalanine ammonia-lyase(PAL)and chalcone synthase(CHS)as well as their cognate transcripts were positively correlated with the variation in the flavonoid content,while shikimate O-hydroxycinnamoyltransferase(HCT)-(encoding genes had the opposite pattern.The increase in the abundance of proteins and mRNAs corresponding to alcohol dehydrogenase(ADH)was associated with a reduction in linoleic acid synthesis.Using weighted gene coexpression network analysis(WGCNA),we further identi fied six unique modules related to flavonoid,oil,and fatty acid anabolism that contained hub genes or p

关 键 词：metabolism analysis MATURITY 

分 类 号：S794.4[农业科学—林木遗传育种]