Dynamic changes in transposable element and gene methylation in mulberry (Morus notabilis) in response to Botrytis cinerea  

作　　者：Youchao Xin Bi Ma Qiwei Zeng Wenmin He Meiling Qin Ningjia He 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology,Southwest University,Beibei,Chongqing 400715,China [2]College of Forestry,Shandong Agricultural University,Taian,Shandong 271018,China

出　　处：《Horticulture Research》2021年第1期2149-2162,共14页园艺研究（英文）

基　　金：This project was funded by the National Key Research and Development Program(No.2018YFD1000602);the Chongqing Research Program of Basic Research and Frontier Technology(cstc2018jcyjAX0407);the Natural Science Foundation of China(No.31572323).

摘　　要：DNA methylation has been proposed to regulate plant stress resistance.However,the dynamic changes in DNA methylation in woody plants and their correlations with pathogenic responses are not fully understood.Here,we present single-base maps of the DNA methylomes of mulberry(Morus notabilis)leaves that were subjected to a mock treatment or inoculation with Botrytis cinerea.Compared with the former,the latter showed decreased mCG and mCHG levels and increased mCHH levels.DNA methylation inhibitors reduced resistance gene methylation levels and enhanced mulberry resistance,suggesting that the hypomethylation of resistance genes affects mulberry resistance to B.cinerea.Virus-induced gene silencing of MnMET1 enhanced the expression of mulberry-resistance genes,thereby increasing the plant’s resistance to B.cinerea.We also found that MITEs play a dominant role in controlling DNA methylation levels.MITEs appear to be the main sources of 24-nt siRNAs that regulate gene expression through the RNA-directed DNA methylation pathway.

关 键 词：RESISTANCE thereby latter 

分 类 号：Q94[生物学—植物学]