Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin depolymerizing factor 1 on microfilaments in okra  

作　　者：Biying Dong Qing Yang Zhihua Song Lili Niu Hongyan Cao Tengyue Liu Tingting Du Wanlong Yang Meng Qi Ting Chen Mengying Wang Haojie Jin Dong Meng Yujie Fu 

机构地区：[1]Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,College of Forestry,Beijing Forestry University,Beijing 100000,China [2]Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150000,China

出　　处：《Horticulture Research》2021年第1期2227-2240,共14页园艺研究（英文）

基　　金：The auth ors gratefully ack no wledge the fi nan cial supp ort of the"Nati onal Key R&D Program of China"(2019YFD1000605-1);"The National Natural Science Foun dati on of Ch ina"(31922058);"Outsta nding Young Tale nt Fund in Beij ing Forestry University"(2019JQ03009);"The National Natural Science Foundation of China"(31800509),(31901281),(31930076);"The 111 Project"(B20088);"Heilongjiang Touyan Innovation Team Program"(Tree Genetics and Breeding Innovation Team).

摘　　要：Mature pollen germinates rapidly on the stigma,extending its pollen tube to deliver sperm cells to the ovule for fertilization.The success of this process is an important factor that limits output.The flavonoid content increased signi ficantly during pollen germination and pollen tube growth,which suggests it may play an important role in these processes.However,the speci fi c mechanism of this involvement has been little researched.Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus(okra),but its speci fic mechanism is still unclear.Therefore,in this study,we focused on the effect of hyperoside in regulating the actin-depolymerizing factor(ADF),which further affects the germination and growth of pollen.We found that hyperoside can prolong the effective pollination period of okra by 2-3-fold and promote the growth of pollen tubes in the style.Then,we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular micro fi laments.Hyperoside can promote pollen germination and pollen tube elongation in vitro.Moreover,AeADFl was identi fied out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside.In addition,hyperoside promoted AeADF1-mediated micro filament dissipation according to micro filament severing experiments in vitro.In the pollen tube,the gene expression of AeADFl was reduced to 1/5 by oligonucleotide transfection.The decrease in the expression level of AeADFl partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth.This research provides new research directions for flavonoids in reproductive development.

关 键 词：EFFECT POLYMERIZATION polymer 

分 类 号：Q94[生物学—植物学]