Down regulated expression of S_(2)-RNase attenuates self-incompatibility in “Guiyou No.1”pummelo  

作　　者：ianbing Hu Qiang Xu Chenchen Liu Binghao Liu Chongling Deng Chuanwu Chen Zhuangmin Wei Muhammad Husnain Ahmad Kang Peng Hao Wen Xiangling Chen Peng Chen Robert MLarkin Junli Ye Xiuxin Deng Lijun Chai 

机构地区：[1]Key Lab oratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,People's Republic of China [2]Guangxi Engineering Research Center of Citrus Breeding and Culture,Guangxi Academy of Specialty Crops,Guilin 541004,People's Republic of China [3]Guangxi Subtropical Crops Research Institute,Nanning 530001,People's Republic of China [4]Horticulture Research Institute,Guangxi Academy of Agriculture Sciences,Nanning Investigation&Experiment Station of South Subtropical Fruit Trees,Ministry of Agriculture,Nanning 530007 Guangxi,People's Republic of China [5]Horticultural Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,People's Republic of China

出　　处：《Horticulture Research》2021年第1期2585-2599,共15页园艺研究（英文）

基　　金：This research was financially supported by the National Key Research and Development Program of China(grant no.2018YFD1000107);the National Natural Science Foundation of China(grant nos.31772259,31630065,and 31521092);the Fundamental Research Funds forthe Central Univer sities(grant no.2662019PY044);the China Agriculture Research System of MOF and MARA and the Hubei Provincial Natural Science Foundation of China(2020CFB532).

摘　　要：Self-incompatibility(SI)substantially restricts the yield and quality of citrus.Therefore,breeding and analyzing selfcompatible germplasm is of great theoretical and practical signi ficance for citrus.Here,we focus on the mechanism of a self-compatibility mutation in‘Guiyou No.1'pummelo(Citrus maxima),which is a spontaneous mutant of‘Shatian’pummelo(Citrus maxima,self-incompatibility).The rate of fruit set and the growth of pollen tubes in the pistil con firmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of‘Guiyou No.1'.Segregation ratios of the S genotype in progeny,expression analysis,and western blotting validated that the reduced levels of S_(2)-RNase mRNA contribute to the loss of SI in‘Guiyou No.1'.Furthermore,we report a phased assembly of the‘Guiyou No.1'pummelo genome and obtained two complete and well-annotated S haplotypes.Coupled with an analysis of SV variations,methylation levels,and gene expression,we identi fied a candidate gene(CgHB40),that may in fluence the regulation of the S/^RNase promoter.Our data provide evidence that a mutation that affects the pistilled to the loss of SI in‘Guiyou No.1'by in fluencing a poorly understood mechanism that affects transcriptional regulation.This work signi ficantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes.

关 键 词：CITRUS No.1 compatibility 

分 类 号：S666.3[农业科学—果树学]