A high-quality chromosome-scale assembly of the centipedegrass[Eremochloa ophiuroides(Munro)Hack.]genome provides in sights into chromosomal structural evolution and prostrate growth habit  

作　　者：Jingjing Wang Hailing Zi Rui Wang Jianxiu Liu Haoran Wang Rongrong Chen Ling Li Hailin Guo Jingbo Chen Jianjian Li Junqin Zong 

机构地区：[1]The National Forestry and Grassland Administration Engineering Research Center for Germplasm Innovation and Utilization of Warm-season Turfgrasses,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China [2]Novogene Bioinformatics Institute,Beijing,China

出　　处：《Horticulture Research》2021年第1期2624-2636,共13页园艺研究（英文）

基　　金：This work was funded by the National Natural Science Foundation of China[Grant No.31902060,31771870,31902046,32072608];the Natural Science Foundation of Jiangsu Province,China[Grant No.BK20180315,BK20200288];the Jiangsu Agricultural Science and Tech no logy Inno vatio n Fund[Grant No.CX(20)3141];the Open Fund of Jia ngsu Provin cial Key Lab oratory for the Resea rch and Utilizati on of Pla nt Resource,China[Grant No.JSPKLB201840,JSPKLB201817,JSPKLB201927].

摘　　要：Centipedegrass[Eremochloa ophiuroides(Munro)Hack.],a member of the Panicoideae subfamily,is one of the most important warm-season turfgrasses originating from China.This grass has an extremely developed prostrate growth habit and has been widely used in transitional and warm climatic regions.To better understand the genetic basis of important biological characteristics,such as prostrate growth and seed yield,in warm-season turfgrasses,we present a high-quality reference genome for centipedegrass and use PacBio,BioNano,and Hi-C technologies to anchor the 867.43 Mb genome assembly into nine pseudochromosomes,with a scaffold N50 of 86.05 Mb and 36,572 annotated genes.Centipedegrass was most closely related to sorghum and diverged from their common ancestor~16.8 Mya.We detected a novel chromosome reshuf fling event in centipedegrass,namely,the nest chromosome fusion event in which fusion of chromosomes 8 and 10 of sorghum into chromosome 3 of centipedegrass likely occurred after the divergence of centipedegrass from sorghum.The typical prostrate growth trait in centipedegrass may be linked to the expansion of candidate PROSTRATE GROWTH 1(PROG1)genes on chromosome 2.Two orthologous genes of OsPROGl,EoPROGl,and EoPROG2,were con firmed to increase the stem number and decrease the stem angle in Arabidopsis.Collectively,our assembled reference genome of centipedegrass offers new knowledge and resources to dissect the genome evolution of Panicoideae and accelerate genome-assisted breeding and improvement of plant architecture in turf plants.

关 键 词：breeding FIR evolution 

分 类 号：Q943.2[生物学—植物学]