Development of potent promoters that drive the efficient expression of genes in apple protoplasts  

作　　者：Xianpu Wang Lili Xu Xiuxia Liu Li Xin Shujing Wu Xuesen Chen 

机构地区：[1]College of Horticultural Science and Engineering,State Key Laboratory of Crop Biology,Shandong Agricultural University,Tai’an,Shandong,PR China

出　　处：《Horticulture Research》2021年第1期2872-2880,共9页园艺研究（英文）

基　　金：This work was supported by grants from the National Key Research and Development Program of China(2018YFD1000307);the Natural Science Foundation in China(nos.31672136 and 31272132).

摘　　要：Protoplast transient expression is a powerful strategy for gene functional characterization,especially in biochemical mechanism studies.We herein developed a highly efficient transient expression system for apple protoplasts.The abilities of the Arabidopsis thaliana and Malus domestica ubiquitin-10(AtUBQ10 and MdUBQ10)promoters to drive the expression of multiple genes were compared with that of the CaMV 35S promoter,and the results revealed that the AtUBQ10 and MdUBQ10 promoters were more efficient in apple protoplasts.With this system,we demonstrated that active AtMKK7ac could activate MAPK6/3/4 signaling cascades,which further regulated MdWRKY33 phosphorylation and stability in apple.Furthermore,the ligand-induced interaction between the immune receptor AtFLS2 and the coreceptor AtBAK1 was reconstituted in apple protoplasts.We also found that the stability of the bacterial effector AvrRpt2 was regulated by feedback involving auxin and the immune regulator RIN4.The system established herein will serve as a useful tool for the molecular and biochemical analyses of apple genes.

关 键 词：herein TRANSIENT stability 

分 类 号：S661.1[农业科学—果树学]