MdWRKY75e enhances resistance to Alternaria alternata in Malus domestica  

作　　者：Yingjun Hou Xinyi Yu Weiping Chen Weibing Zhuang Sanhong Wang Chao Sun Lifang Cao Tingting Zhou Shenchun Qu 

机构地区：[1]College of Horticulture,Nanjing Agricultural University,Nanjing,People's Republic of China [2]College of Agriculture,Nanjing Agricultural University,Nanjing,People's Republic of China [3]Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden,Memorial Sun Yat-sen),Nanjing,People's Republic of China

出　　处：《Horticulture Research》2021年第1期3068-3088,共21页园艺研究（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(grant number 31872074);the National Key R&D Program of China(2019YFD1000100);Priority Academic Program Development of Jiangsu Higher Education Institutions。

摘　　要：The Alternaria alternata apple pathotype adversely affects apple(Malus domestica Borkh.)cultivation.However,the molecular mechanisms underlying enhanced resistance to this pathogen in apple remain poorly understood.We have previously reported that MdWRKY75 expression is upregulated by A.alternata infection in‘Sushuai’apples.In this study,we discovered that overexpression of MdWRKY75e increased the resistance of transgenic apple lines to A.alternata infection,whereas silencing this gene enhanced susceptibility to A.alternata infection.Furthermore,we found that MdWRKY75e directly binds to the MdLAC7 promoter to regulate the biosynthesis of laccase and increase the biosynthesis of lignin during A.alternata infection.Moreover,the thickening of the cell wall enhanced the mechanical defense capabilities of apple.In addition,we found that jasmonic acid remarkably induced MdWRKY75e expression,and its levels in transgenic apple lines were elevated.These results indicate that MdWRKY75e confers resistance to the A.alternata apple pathotype mainly via the jasmonic acid pathway and that pathogenesis-related genes and antioxidant-related enzyme activity are involved in the disease resistance of MdWRKY75e transgenic plants.In conclusion,our fi ndings provide insights into the importance of MdWRKY75e for resistance to A.alternata infection in apples.

关 键 词：ALTERNATA APPLE domestica 

分 类 号：S43[农业科学—农业昆虫与害虫防治]