Characterization of germline development and identification of genes associated with germline specification in pineapple  

作　　者：Lihua Zhao Liping Liu Yanhui Liu Xianying Dou Hanyang Cai Mohammad Aslam Zhimin Hou Xingyue Jin Yi Li Lulu Wang Heming Zhao Xiaomei Wang Adrien Sicard Yuan Qin 

机构地区：[1]College of Life Science,State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,Center for Genomics and Biotechnology,Fujian Agricult ure and Forstry University,Fuzhou,China [2]Department of Plant Biology,Swedish University of Agricultural Sciences,Uppsala BioCenter and Linnean Centre for Plant Biology,Uppsala,Sweden [3]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi Key Lab of Sugarcane Biology,College of Agriculture,Guangxi University,Nanning,Guangxi,China [4]Horticulture Research Institute,Guangxi Academy of Agricultural Sciences,Nanning Investigation Station of South Subtropical Fruit Trees,Ministry of Agriculture,Nanning,China

出　　处：《Horticulture Research》2021年第1期3172-3192,共21页园艺研究（英文）

基　　金：This work was supported by the Science and Technology Program of Fujian Province(2019N5008);the Science and Technology Major Project of Guangxi(Gui Ke 2018-266-Z01);the National Natural Science Foundation of China(31970333,U1605212);a Guangxi Distinguished Experts Fellowship awarded to Y.Q;a Science and Technology Major Project of Guangxi-Research and application of ecological and highly efficient cultivation techniques for dominant and characteristic fruits(AA17204097-6)awarded to X.W.

摘　　要：Understanding germline specification in plants could be advantageous for agricultural applications.In recent decades,substantial efforts have been made to understand germline speci fication in several plant species,including Arabidopsis,rice,and maize.However,our knowledge of germline speci fication in many agronomically important plant species remains obscure.Here,we characterized the female germline speci fication and subsequent female gametophyte development in pineapple using callose staining,cytological,and whole-mount immunolocalization analyses.We also determined the male germline speci fication and gametophyte developmental timeline and observed male meiotic behavior using chromosome spreading assays.Furthermore,we identi fied 229 genes that are preferentially expressed at the megaspore mother cell(MMC)stage during ovule development and 478 genes that are preferentially expressed at the pollen mother cell(PMC)stage of anther development using comparative transcriptomic analysis.The biological functions,associated regulatory pathways and expression patterns of these genes were also analyzed.Our study provides a convenient cytological reference for exploring pineapple germline development and a molecular basis for the future functional analysis of germline speci fi cation in related plant species.

关 键 词：SPECIES MOTHER MOUNT 

分 类 号：S668.3[农业科学—果树学]