Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants  

作　　者：Guoliang Yuan Haiwei Lu Dan Tang Md Mahmudul Hassan Yi Li Jin-Gui Chen Gerald A.Tuskan Xiaohan Yang 

机构地区：[1]Bioscie nces Divisi on,Oak Ridge Nati onal Lab oratory,Oak Ridge,TN 37831,USA [2]The Center for Bioe nergy Inno vati on,Oak Ridge Nati onal Lab oratory,Oak Ridge,TN 37831,USA [3]Department of Plant Science and Landscape Architecture,University of Connecticut,Storrs,CT 06269,USA [4]National Center for Citrus Improvement,College of Horticulture,Hunan Agricultural University,Changsha 410128 Hunan,China [5]Department of Genetics and Plant Breeding,Patuakhali Science and Technology University,Dumki,Patuakhali 8602,Bangladesh

出　　处：《Horticulture Research》2021年第1期3286-3296,共11页园艺研究（英文）

摘　　要：Green fl uorescent protein(GFP)has been widely used for monitoring gene expression and protein localization in diverse organisms.However,highly sensitive imaging equipment,like fl uorescence microscope,is usually required for the visualization of GFP,limitings its application to fi xed locations in samples.A reporter that can be visualized in realtime regardless the shape,size and location of the target samples will increase the fl exibility and ef fi ciency of research work.Here,we report the application of a GFP-like protein,called eYGFPuv,in both transient expression and stable transformation,in two herbaceous plant species(Arabidopsis and tobacco)and two woody plant species(poplar and citrus).We observed bright fl uorescence under UV light in all of the four plant species without any effects on plant growth or development.eYGFPuv was shown to be effective for imaging transient expression in leaf and root tissues.With a focus on in vitro transformation,we demonstrated that the transgenic events expressing 1x eYGFPuv could be easily identi fi ed visually during the callus stage and the shoot stage,enabling early and ef fi cient selection of transformants.Furthermore,whole-plant level visualization of eYGFPuv revealed its ubiquitous stability in transgenic plants.In addition,our transformation experiments showed that eYGFPuv can also be used to select transgenic plants without antibiotics.This work demonstrates the feasibility of utilizing 1x eYGFPuv in studies of gene expression and plant transformation in diverse plants.

关 键 词：TRANSIENT TRANSFORMATION application 

分 类 号：Q94[生物学—植物学]