Integrative genome,transcriptome,microRNA,and degradome analysis of water dropwort(Oenanthe javanica)in response to water stress  

作　　者：Jie-Xia Liu Qian Jiang Jian-Ping Tao Kai Feng Tong Li Ao-Qi Duan Hao Wang Zhi-Sheng Xu Hui Liu Ai-Sheng Xiong 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,College of Horticulture,Nanjing Agricultural University,1 Weigang,210095 Nanjing,China

出　　处：《Horticulture Research》2021年第1期3396-3412,共17页园艺研究（英文）

基　　金：This research was supported by Jiangsu Agricultural Science and Technology Innovation Fund(CX(18)2007);New Century Excellent Talents in University(NCET-11-0670);National Natural Science Foundation of China(31272175);Priority Academic Program Development of Jiangsu Higher Education Institutions Project(PAPD).

摘　　要：Water dropwort(Liyang Baiqin,Oenanthe javanica(BI.)DC.)is an aquatic perennial plant from the Apiaceae family with abundant protein,dietary fiber,vitamins,and minerals.It usually grows in wet soils and can even grow in water.Here,whole-genome sequencing of O.javanica via HiSeq 2000 sequencing technology was reported for the first time.The genome size was 1.28 Gb,including 42,270 genes,of which 93.92%could be functionally annotated.An online database of the whole-genome sequences of water dropwort,Water dropwortDB,was established to share the results and facilitate further research on O.javanica(database homepage:http://apiaceae.njau.edu.cn/waterdropwortdb).Water dropwortDB offers whole-genome and transcriptome sequences and a Basic Local Alignment Search Tool.Comparative analysis with other species showed that the evolutionary relationship between O.javanica and Daucus carota was the closest.Twenty-five gene families of O.javanica were found to be expanded,and some genetic factors(such as genes and miRNAs)related to phenotypic and anatomic differentiation in O.javanica under different water conditions were further investigated.Two miRNA and target gene pairs(miR408 and Oja15472,miR171 and Oja47040)were remarkably regulated by water stress.The obtained reference genome of O.javanica provides important information for future work,thus making in-depth genetic breeding and gene editing possible.The present study also provides a foundation for the understanding of the O.javanica response to water stress,including morphological,anatomical,and genetic differentiation.

关 键 词：VITAMIN stress ANALYSIS 

分 类 号：Q94[生物学—植物学]