Organelle genome assembly uncovers the dynamic genome reorganization and cytoplasmic male sterility associated genes in tomato  

作　　者：Kosuke Kuwabara Issei Harada Yuma Matsuzawa Tohru Ariizumi Kenta Shirasawa 

机构地区：[1]Graduate School of Life and Environmental Sciences,University of Tsukuba,Tsukuba,Ibaraki 305-8577,Japan [2]TOKITA Seed Co.LTD.,Kazo,Saitama 349-1144,Japan [3]Tsukuba Plant Innovation Research Center,Tsukuba,Ibaraki 305-8577,Japan [4]Kazusa DNA Research Institute,Kisarazu,Chiba 292-0818,Japan

出　　处：《Horticulture Research》2021年第1期3464-3474,共11页园艺研究（英文）

基　　金：This work was supported by the Kazusa DNA Research Institute Foundation to K.S.;the Project of the NARO Bio-oriented Technology Research Advancement Institution(Research Program on Development of Innovative Technology,Grant numbers:30010A and ID21448196)to K.S.,Y.M.;T.A.,JSPS KAKENHI(Grant Numbers:17H03761 and 21H02181)to T.A.;JSPS Research Fellowships for Young Researchers(Grant Number:21J20479)to K.K;Seeds of Micro-Tom(TOMJPF0001)was provided from National BioResource Project Tomato(NBRP tomato).

摘　　要：To identify cytoplasmic male sterility(CMS)-associated genes in tomato,we determined the genome sequences of mitochondria and chloroplasts in three CMS tomato lines derived from independent asymmetric cell fusions,their nuclear and cytoplasmic donors,and male fertile weedy cultivated tomato and wild relatives.The structures of the CMS mitochondrial genomes were highly divergent from those of the nuclear and cytoplasmic donors,and genes of the donors were mixed up in these genomes.On the other hand,the structures of CMS chloroplast genomes were moderately conserved across the donors,but CMS chloroplast genes were unexpectedly likely derived from the nuclear donors.Comparative analysis of the structures and contents of organelle genes and transcriptome analysis identified three genes that were uniquely present in the CMS lines,but not in the donor or fertile lines.RNA-sequencing analysis indicated that these three genes transcriptionally expressed in anther,and identified different RNA editing levels in one gene,orf265,that was partially similar to ATP synthase subunit 8,between fertile and sterile lines.The orf265 was a highly potential candidate for CMS-associated gene.This study suggests that organelle reorganization mechanisms after cell fusion events differ between mitochondria and chloroplasts,and provides insight into the development of new F1 hybrid breeding programs employing the CMS system in tomato.

关 键 词：breeding STERILE DONOR 

分 类 号：Q94[生物学—植物学]