Genome-wide unbalanced expression bias and expression level dominance toward Brassica oleracea in artificially synthesized intergeneric hybrids of Raphanobrassica  

作　　者：Libin Zhang Jianjie He Hongsheng He Jiangsheng Wu Maoteng Li 

机构地区：[1]College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China [2]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Horticulture Research》2021年第1期3611-3622,共12页园艺研究（英文）

基　　金：the National Key Research and Development Program of China(2017YFD0101701);the National Natural Science Foundation of China(Grant No.31540083).

摘　　要：Raphanobrassica(R^(r)R^(r)C^(r)C^(r),2n=4x=36),which is generated by distant hybridization between the maternal parent Raphanus sativus(R s R s,2n=2x=18)and the paternal parent Brassica oleracea(C°C°,2n=2x=18),displays intermediate silique phenotypes compared to diploid progenitors.However,the hybrid shares much more similarities in silique phenotypes with those of B.oleracea than those of R.sativus.Strikingly,the silique of Raphanobrassica is obviously split into two parts.To investigate the gene expression patterns behind these phenomena,transcriptome analysis was performed on the upper,middle,and lower sections of pods(RCsiu,RCsim,and RCsil),seeds in the upper and lower sections of siliques(RCseu and RCsel)from Raphanobrassica,whole pods(Rsi and Csi)and all seeds in the siliques(Rse and Cse)from R.sativus and B.oleracea.Transcriptome shock was observed in all five aforementioned tissues of Raphanobrassica.Genome-wide unbalanced biased expression and expression level dominance were also discovered,and both of them were toward B.oleracea in Raphanobrassica,which is consistent with the observed phenotypes.The present results reveal the global gene expression patterns of different sections of siliques of Raphanobrassica,pods,and seeds of B.oleracea and R.sativus,unraveling the tight correlation between global gene expression patterns and phenotypes of the hybrid and its parents.

关 键 词：DOMINANCE mentioned SECTIONS 

分 类 号：S334[农业科学—作物遗传育种]