对氯苯甲酸构筑的铜(Ⅱ)配合物的合成、HSA结合及细胞毒性  

Synthesis, HSA Binding, and Cytotoxic Activity of Copper(Ⅱ) Complex Constructed by P-Chlorobenzoic Acid

作　　者：曾振芳[1] 袁芳[1] 黄秋萍[1] 庞华钰 杨红兰[1] 黄秋婵[1] ZENG Zhenfang;YUAN Fang;HUANG Qiuping;PANG Huayu;YANG Honglan;HUANG Qiuchan(School of Chemical and Biological Engineering,Guangxi Normal University for Nationalities,Chongzuo 532200,China)

机构地区：[1]广西民族师范学院化学与生物工程学院,崇左532200

出　　处：《人工晶体学报》2022年第1期126-131,共6页Journal of Synthetic Crystals

基　　金：广西自然科学基金(2020JJB130043);广西教育厅高校科研项目(2017KY0846);广西民族师范学院科研项目(2020JS002,2021YB036,2021YB038,2019YB018,2020GP008)。

摘　　要：本文合成了配合物[Cu(pcba)_(2)·(phen)(H_(2) O)](pcba=对氯苯甲酸,phen=1,10-邻菲罗啉),该配合物属于三斜晶系,P1空间群,晶胞参数为a=0.79098(2)nm,b=1.07240(4)nm,c=1.48719(6)nm,α=100.613(3)°,β=95.239(3)°,γ=108.334(3)°,Z=2,D c=1.638 g·cm^(-3),F(000)=582,最终结构残差因子R_(1)=0.0359,wR_(2)=0.0891。采用紫外及荧光研究了配合物和人血清蛋白(HSA)的相互作用方式。结果表明,配合物静态猝灭HSA荧光,可求得配合物与HSA的猝灭常数K_(sv)=2.35×10^(5)L·mol^(-1),猝灭速率常数K_(q)=2.35×10^(13) L·mol^(-1)·s^(-1),结合常数为K_(a)=2.14×10^(13)L·mol^(-1),结合位点n=2.37。同时,研究了配合物对胃癌细胞A549、宫颈癌细胞Hela和肝癌细胞HepG2的抗增殖能力。In this paper,a copper(Ⅱ)complex[Cu(pcba)_(2)·(phen)(H_(2) O)](pcba=p-chlorbenzoic acid,phen=1,10-phenanthroline)was synthesized.The structure of the complex belongs to triclinic,P1 space group,with a=0.79098(2)nm,b=1.07240(4)nm,c=1.48719(6)nm,α=100.613(3)°,β=95.239(3)°,γ=108.334(3)°,Z=2,D c=1.638 g·cm^(-3),F(000)=582 unit cell parameters.The final R_(1)=0.0359,wR_(2)=0.0891.The interaction between HSA(human serum albumin)and the complex was evaluated by UV-Vis and fluorescence spectroscopy.The complex quenched the intrinsic fluorescence of HSA via static quenching with K_(sv)=2.35×10^(5)L·mol^(-1),the quenching rate constant K_(q)=2.35×10^(13) L·mol^(-1)·s^(-1),the binding constant K_(a)=2.14×10^(13)L·mol^(-1),the binding site n=2.37.Furthermore,the proliferation inhibition effect of the complex on the cells A549,Hela and HepG2 was studied.

关 键 词：配合物 铜金属配合物 人血清蛋白 对氯苯甲酸 铜(Ⅱ) HSA结合 细胞毒性 

分 类 号：O614.4[理学—无机化学] TQ460.1[理学—化学]