凉血固元益肾汤对肠型急性辐射损伤大鼠骨髓造血功能的保护作用  

Protective Effect of Liangxue Guyuan Yishen Decoction on Bone Marrow Hematopoietic Function in Rats with Intestinal Acute Radiation Injury

作　　者：闫梓乔 王毓国[2] 冯剑 杨倩宇 窦永起 YAN Zi-qiao;WANG Yu-guo;FENG Jian;YANG Qian-yu;DOU Yong-qi(Medical College of Chinese People's Liberation Army,Beijing 100853;Department of Traditional Chinese Medicine,General Hospital of Chinese People's Liberation Army,Beijing 100048;Hebei University of Traditional Chinese Medicine,Shijiazhuang Hebei 050000)

机构地区：[1]解放军医学院,北京100853 [2]解放军总医院中医医学部,北京100048 [3]河北中医学院,河北石家庄050000

出　　处：《世界中西医结合杂志》2021年第12期2232-2237,2260,共7页World Journal of Integrated Traditional and Western Medicine

基　　金：全军后勤科研计划项目(AWS14C014)。

摘　　要：目的通过动物实验观察凉血固元益肾汤对肠型急性辐射损伤大鼠骨髓造血机能的保护作用。方法清洁级雄性SD大鼠85只,随机分为空白对照组、模型对照组、阳性药对照组、凉血固元益肾汤中剂量组(中药中剂量组)、凉血固元益肾汤高剂量组(中药高剂量组)。在适应性饲养后,于照射前禁食8 h,除空白对照组外,各组均通过全身一次性^(60)Co-γ射线10 Gy(源皮距3 m)照射制备大鼠急性肠型辐射损伤模型。自照射后第1天至处死前,中药中、高剂量组分别按照10 ml/kg给予含生药2.73 g/ml、5.46 g/ml的凉血固元益肾汤浓缩液灌胃,阳性药对照组按10 ml/kg给予生理盐水配置的0.3 g/ml谷氨酰胺混悬液灌胃,空白对照组和模型对照组按10 ml/kg给予生理盐水灌胃,1次/d。在照射后第3、5、10天,除空白对照组外各组分别随机抽取5只检测白细胞、红细胞(红细胞计数、血红蛋白浓度和红细胞比容)、血小板情况,并剖取一侧股骨进行骨髓病理切片染色观察。结果(1)白细胞:与空白对照组比较,各组在照射后第3、5、10天均明显降低(P<0.01),各治疗组与模型对照组及各治疗组组间比较,差异无统计学意义(P>0.05)。(2)红细胞:照射后第3天,与空白对照组比较,模型对照组红细胞计数、血红蛋白浓度和红细胞比容明显降低(P<0.05,P<0.01),中药高剂量组各指标高于模型对照组及阳性药对照组(P<0.01),甚至其红细胞计数和血红蛋白浓度高于空白对照组(P<0.05);照射后第5天,各组红细胞计数、血红蛋白浓度和红细胞比容比较,差异无统计学意义(P>0.05)。照射后第10天,与空白对照组比较各组红细胞计数、血红蛋白浓度和红细胞比容均出现明显降低(P<0.01),且中药中剂量组红细胞计数和红细胞比容水平均高于模型对照组(P<0.05)。(3)血小板:照射后第3天,各组血小板计数比较,差异无统计学意义(P>0.05),但第5天和第10天各组血�Objective To observe the protective effect of Liangxue Guyuan Yishen Decoction(LXGYD)on bone marrow hematopoietic function in rats with Gastrointestinal-Acute Radiation injury Syndrome(GI-ARS).Methods Eighty-five male SD rats were randomly divided into blank control group,model control group,positive drug control group,Liangxue Guyuan Yishen Decoction medium dose group(Chinese medicine medium dose group),Liangxue GuyuanYishen Decoction high-dose group(Chinese medicine high-dose group).After adaptive feeding for 3 days,rats were fasted at 8 h before radiation.Except for the Control group,rats in each group were irradiated by whole-body one-time 10 Gy^(60)Co-γray(source dermal distance is 3 m)to prepare the experimental animal model of GI-ARS.From d1 after radiation to the day before death,the M D and H D group were respectively given a concentrated solution of water decocted TCM containing crude drugs at 10 ml/kg,the Glu group was given 0.3 g/ml glutamine suspension at 10 ml/kg,and the Control group and Radiation group were given normal saline at 10 ml/kg.Gavage was once a day.After irradiation on D3,D5 and D10,except for the Control group,5 rats were randomly selected from each group to detect the counts of RBC,WBC and PLT in peripheral blood,and bone marrow pathological sections were stained for observation.Results(1)White blood cells:Compared with the blank control group,each group was significantly reduced on the 3;,5;,and 10;day after irradiation(P<0.01).There were differences between the treatment groups and the model control group and the treatment groups.No statistical significance(P>0.05).(2)Red blood cells:On the 3;day after irradiation,compared with the blank control group,the red blood cell count,hemoglobin concentration and hematocrit of the model control group were significantly reduced(P<0.05,P<0.01),and the indicators of the high-dose Chinese medicine group were higher than those of the model The red blood cell count and hemoglobin concentration of the control group and the positive drug control g

关 键 词：中药复方 肠型急性辐射损伤 急性辐射损伤综合征 骨髓 造血 

分 类 号：R285.5[医药卫生—中药学]