葛根异黄酮对高尿酸血症小鼠炎症、应激反应的影响研究  

Effect of Pueraria Isoflavones on Inflammation and Stress Response in Hyperuricemia Mice

作　　者：李桐 朱天麒 方少婷 欧阳军艳 向惠芳 陈赛珍 王耀霆 姚雨涵 黄春霞[1] LI Tong;ZHU Tianqi;FANG Shaoting;OUYANG Junyan;XIANG Huifang;CHEN Saizhen;WAGN Yaoting;YAO Yuhan;HUANG Chunxia(Changsha Medical College,Changsha 410219,China)

机构地区：[1]长沙医学院,湖南省长沙市410219

出　　处：《实用心脑肺血管病杂志》2022年第2期75-80,共6页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：国家级大学生创新创业训练计划项目(S202010823014);2020年度湖南省大学生创新创业训练计划项目(3897,1087)。

摘　　要：背景人类因缺乏尿酸酶基因而无法代谢不溶性尿酸(UA),而高嘌呤饮食、代谢疾病均会导致UA升高,病情严重者可诱发肾功能不全等疾病。黄酮类化合物对多种酶有抑制作用,异黄酮是中药葛根的主要功效成分,其已被证实具有明确的降糖、降脂作用。目的探讨葛根异黄酮对高尿酸血症(HUA)小鼠炎症、应激反应的影响。方法2020年12月至2021年6月,采用随机数字表法将48只SPF级昆明小鼠分为正常组、模型组、条件对照组、实验组,各12只。正常组小鼠每天灌胃0.5%羧甲基纤维素钠;其余三组小鼠每天灌胃等体积腺嘌呤和盐酸乙胺丁醇混合液以制备HUA模型,共计30 d。造模成功后正常组小鼠继续用混合饲料饲养,不予其他药物;模型组小鼠继续灌胃0.5%羧甲基纤维素钠,不予其他药物;条件对照组小鼠继续灌胃0.5%羧甲基纤维素钠,同时灌胃非布司溶液;实验组小鼠继续灌胃0.5%羧甲基纤维素钠,同时灌胃葛根异黄酮;四组小鼠干预时间均为30 d。比较四组小鼠干预前后血清UA、炎症指标〔包括P物质(SP)、红细胞沉降率(ESR)、C反应蛋白(CRP)、肿瘤坏死因子α(TNF-α)、白介素6(IL-6)〕、应激反应指标〔包括超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)、丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(NOS)〕。观察四组小鼠主动脉弓内皮细胞病理学特征。结果干预后,模型组、条件对照组、实验组小鼠血清UA高于正常组,条件对照组、实验组小鼠血清UA低于模型组(P<0.05);模型组、条件对照组、实验组小鼠干预后血清UA分别高于本组干预前(P<0.05)。干预后,模型组、条件对照组、实验组小鼠血清SP、ESR、CRP、TNF-α高于正常组,条件对照组、实验组小鼠血清SP、ESR、CRP、TNF-α低于模型组,实验组小鼠血清SP、ESR、CRP低于条件对照组(P<0.05);模型组、条件对照组、实验组小鼠干预后血清SP、ESR、CRP、TNF-Background Humans cannot metabolize insoluble uric acid(UA)due to the lack of uricase gene,and high purine diet and metabolic diseases can lead to increased UA,and severe cases can induce diseases such as renal insufficiency.Flavonoids have inhibitory effects on a variety of enzymes,isoflavones are the main functional components of traditional Chinese medicine Pueraria,which have been confirmed to have clear hypoglycemic and lipid-lowering effects.Objective To investigate the effect of pueraria isoflavones on inflammation and stress response in hyperuricemia(HUA)mice.Methods From December 2020 to June 2021,48 SPF Kunming mice were divided into normal group,model group,condition control group,and experimental group by random number table method,with 12 mice in each group.The mice in the normal group were given 0.5%sodium carboxymethyl cellulose by gavage every day;the mice in the other three groups were given an equal volume of a mixture of adenine and ethambutol hydrochloride by gavage every day to make the HUA model,for a total of 30 d.After successful modeling,the mice in the normal group were fed with mixed feed without other drugs;the mice in the model group were continuously gavaged with 0.5%carboxymethyl cellulose sodium without other drugs;the mice in the condition control group were continuously gavaged with 0.5%carboxymethyl cellulose sodium and fibrate solution at the same time;the mice in the experimental group were gavaged with 0.5%carboxymethyl cellulose sodium and pueraria isoflavones at the same time;the intervention time of the four groups was 30 d.Serum UA,inflammatory indexes[including substance P(SP),erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),tumor necrosis factor alpha(TNF-α),interleukin 6(IL-6)],and stress response indexes[including superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),nitric oxide(NO),nitric oxide synthase(NOS)]before and after intervention in four groups were compared.The pathological characteristics of aortic arch endothelial cells

关 键 词：高尿酸血症 葛根 异黄酮类 炎症 应激 生理学 

分 类 号：R589.9[医药卫生—内分泌]