Novel pneumatically assisted atomization device for living cell delivery:application of sprayed mesenchymal stem cells for skin regeneration  

作　　者：Lixing Zhang Xintao Yan Li An Meijia Wang Xi Xu Zhonglin Ma Mengting Nie Fangzhou Du Jingzhong Zhang Shuang Yu 

机构地区：[1]Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China [2]Zhengzhou Institute of Engineering and Technology Affiliated with SIBET,Zhengzhou 450001,China [3]Xuzhou Medical University,Xuzhou 221004,China [4]Academy for Engineering and Technology,Fudan University,Shanghai 200433,China

出　　处：《Bio-Design and Manufacturing》2022年第1期220-232,共13页生物设计与制造（英文）

基　　金：the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020807);the Key Research and Development Program of Jiangsu Province,China(Nos.BE2018668 and BE2017669);the Major Innovative Research Team of Suzhou,China(No.ZXT2019007);SIBET and Jilin City Science and Technology Cooperation Project(No.E0550104);Doctor of Entrepreneurship and Innovation Program of Jiangsu Province in the year of 2020.

摘　　要：Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration.However,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration methods.Specifically,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for proliferation.Herein,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery pressure.We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound site.Moreover,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model.Overall,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.

关 键 词：Cell delivery Skin wound Wound healing Spray application Cell-spray autografting Stem cell therapy 

分 类 号：R641[医药卫生—外科学] TH77[医药卫生—临床医学]