茵连化浊解毒汤对胆结石小鼠血生化指标、胆汁代谢谱的影响及其机制分析  

Analysis on the Effect of Yinlian Huazhuo Jiedu Decoction on Blood Biochemical Indicators and Bile Metabolism Spectrum in Gallstone Mice and Its Mechanism

作　　者：张正伟 吴佳玉 李斌 ZHANG Zhengwei;WU Jiayu;LI Bin(Department of Pharmacy,Luoyang Central Hospital Affiliated to Zhengzhou University,Henan Province,Luoyang 741000,China)

机构地区：[1]郑州大学附属洛阳中心医院药学部,河南洛阳471000

出　　处：《光明中医》2022年第2期228-230,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

摘　　要：目的研究茵连化浊解毒汤对胆结石小鼠血生化指标、胆汁代谢谱的影响及机制。方法选择C57BL/6雄性小鼠,随机分为对照组(普通饲料喂养)、模型组(高脂高热量高胆固醇饲料喂养)、实验组(高脂高热量高胆固醇饲料喂养并给予茵连化浊解毒汤干预)。检测血生化指标、胆汁内代谢产物、肝脏内胆汁酸代谢蛋白的表达。结果模型组小鼠血液中总胆红素(TBIL)、直接胆红素(DBIL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)的含量及胆汁内胆汁酸(TBA)、丙氨酸(Gla)、TC、LDL-C的含量均明显高于对照组,血液中高密度脂蛋白胆固醇(HDL-C)的含量及肝脏中Abcb11、Abcc2的表达水平明显低于对照组;实验组小鼠血液中TBIL、DBIL、ALT、AST、TC、HDL-C、LDL-C的含量及胆汁内TBA、Gla、TC、LDL-C的含量均明显低于模型组,Abcb11、Abcc2表达水平明显高于模型组。结论茵连化浊解毒汤用于胆结石小鼠能够通过增加Abcb11、Abcc2的表达来改善肝功能、调节胆汁代谢。Objective To study the effect and mechanism of Yinlian Huazhuo Jiedu decoction on blood biochemical indexes and bile metabolic spectrum in mice with gallstones. Methods C57 BL/6 male mice were randomly divided into control group( fed with common diet),model group( fed with high fat,high calorie and high cholesterol diet) and experimental group( fed with high fat,high calorie and high cholesterol diet and intervened with Yinlian Huazhuo Jiedu decoction). Blood biochemical indexes,metabolites in bile and expression of bile acid metabolic protein in liver were detected. Results The contents of total bilirubin( TBIL),direct bilirubin( DBIL),alanine aminotransferase( ALT),aspartate aminotransferase( AST),total cholesterol( TC),low density lipoprotein cholesterol( LDL-C) in blood and the contents of bile acid( TBA),alanine( Gla),TC and LDL-C in bile of the model group were significantly higher than those in the control group,the levels of high density lipoprotein cholesterol( HDL-C) in blood and the expression levels of Abcb11 and Abcc2 in liver were significantly lower than those in the control group,the contents of TBIL,DBIL,ALT,AST,TC,LDL-C in blood and TBA,Gla,TC and LDL-C in bile of the experimental group were significantly lower than those in the model group,while the contents of HDL-C in blood and the expression levels of Abcb11 and Abcc2 were significantly higher than those in the model group. Conclusion Yinlian Huazhuo Jiedu decoction can improve liver function and regulate bile metabolism by increasing the expression of Abcb11 and Abcc2 in gallstone mice.

关 键 词：胆结石 茵连化浊解毒汤 肝功能 胆汁代谢 三磷酸腺苷结合盒转运子 实验研究 

分 类 号：R285.5[医药卫生—中药学]