一起由诺如病毒GII.13和GII.17混合感染引起聚集性腹泻疫情的分析  

Analysis of an outbreak of clustered diarrhea caused by co-infection with norovirus GII.13 and GII.17

作　　者：孙振璐 邢晓明[2] 牛培华[1] 张瑞卿 高巧 王佶[1] 马学军[1] Sun Zhenlu;Xing Xiaoming;Niu Peihua;Zhang Ruiqing;Gao Qiao;Wang Ji;Ma Xuejun(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Yantai Center for Disease Control and Prevention,Yantai 264003)

机构地区：[1]中国疾病预防控制中心病毒病预防控制研究所,国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206 [2]烟台市疾病预防控制中心,烟台264003

出　　处：《中华实验和临床病毒学杂志》2021年第6期639-642,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：2017年烟台市科技计划项目(2017WS119)。

摘　　要：目的为明确2019年烟台市某高中一起疫情的病原类型、基因型及分子生物学特征分析,为疫情的防控提供依据。方法收集2019年烟台市某高中一起聚集性疫情标本11份,利用荧光定量PCR初步进行型别鉴定,应用RT—PCR和特异型引物对目的基因进行扩增,对诺如病毒序列进行比对和系统进化分析。结果该聚集性疫情病原为诺如病毒,检出诺如病毒GⅡ核酸阳性样品5份,4份为GII.13型,1份为GII.17型,聚合酶-衣壳区序列分析显示此次聚集性疫情的氨基酸序列高度保守。结论初步判断本次疫情是由GII.13和GII.17型诺如病毒混合感染引起的聚集性腹泻疫情,对本次疫情分析有助于我们对诺如病毒的进化、遗传多样性和分布的普遍了解,有助于进一步指导辖区内诺如病毒的防控工作。Objective To clarify the pathogen types,genotypes and molecular biological characteristics of an outbreak in a high school in Yantai city in 2019,and to provide evidence for epidemic prevention and control.Methods Eleven samples were collected from a high school in Yantai city in 2019.Quantitative PCR was used for primary type identification.RT-PCR and specific primers were used to amplify the target genes,and the sequences of norovirus were compared and analyzed for phylogenetic analysis.Results The pathogen of this cluster outbreak was norovirus.Five GII-positive samples of norovirus were detected,4 of which were GII.13 and 1 was GII.17.Sequence analysis of polymerase-capsid region showed that the amino acid sequence of this cluster outbreak was highly conserved.Conclusions This outbreak is a cluster of diarrhea caused by GII.13 and GII.17 norovirus infection.The analysis of this outbreak is helpful to our general understanding of the evolution,genetic diversity and distribution of norovirus,and the surveillance of norovirus in the jurisdiction should be further strengthened.

关 键 词：诺如病毒 腹泻 聚集性 流行病 

分 类 号：R512.5[医药卫生—内科学] R181.8[医药卫生—临床医学]