从1例新型冠状病毒肺炎病例中同时分离到新型冠状病毒和单纯疱疹病毒I型  

2019-nCoV and herpes simplex virus type I were simultaneously isolated from a case of COVID-19

作　　者：张薇[1] 彭晓放 张欢[1] 刘哲[2] 李柏生[1] 黄天鹏[3] 孙长云 邹丽容[1] 梁丽君[1] 郭前方 武婕[1] 李晖[1] 郑焕英[1] Zhang Wei;Peng Xiaofang;Zhang Huan;Liu Zhe;Li Baisheng;Huang Tianpeng;Sun Changyun;Zou Lirong;Liang Lijun;Guo Qianfang;Wu Jie;Li Hui;Zheng Huanying(Guangdong Center for Disease Control and Prevention,Guangzhou 511430,China;Guangdong Center for Disease Control and Prevention,Guangdong Institute of Public Health,Guangzhou 511430,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]广东省疾病预防控制中心,广州511430 [2]广东省疾病预防控制中心,广东省公共卫生研究院,广州511430 [3]内蒙古农业大学兽医学院,呼和浩特010018

出　　处：《中华实验和临床病毒学杂志》2021年第6期714-718,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：广东省防治新型冠状病毒科技攻关专项-新型冠状病毒人间传播与变异机制研究(2020B111112003)。

摘　　要：目的了解1例新型冠状病毒肺炎(Coronavirus Disease 2019,COVID-19)确诊病例的病原学构成。方法将1例COVID-19确诊病例的口咽拭子、血清、鼻咽拭子的标本接种到Vero-E6细胞管进行病毒分离,每日观察细胞病变(cytopathic effect,CPE)情况,待CPE达75%以上收获细胞分离物,反复冻融3次后离心取上清液,应用透射电镜观察获取病毒电镜图片,同时提取病毒核酸,采用二代测序技术进行全基因组序列测序。通过序列进化分析,对获得的病毒株进行鉴定分型。结果本研究从1例COVID-19患者的鼻咽拭子中分离到1株新型冠状病毒(2019 novel coronavirus,2019-nCoV),同时在该患者的1份口咽拭子中分离到1株单纯疱疹病毒I型(herpes simplex virus type 1,HSV-1)。结论COVID-19病例存在2019-nCoV和HSV-1合并感染的可能性。Objective To understand the etiology of a confirmed case of Coronavirus Disease 2019(COVID-19).Methods The pharyngeal swabs,serum and nasal swabs of a case of COVID-19 were inoculated into Vero-E6 cell tubes for virus isolation.The cytopathic effect(CPE)were observed daily.Collecting cell’s isolation when CPE was over 75%,after repeated freezing and thawing for 3 times,the supernatant was centrifugally taken,and the images of the virus were obtained by transmission electron microscopic observation,and the nucleic acid of the virus was extracted,second generation sequencing and sequence evolution analysis were used to identify and type the virus strains.Results One strain of 2019 novel coronavirus(2019-nCoV)was successfully isolated from the nasal swab of this case of COVID-19,and one strain of herpes simplex virus type 1(HSV-1)was also successfully isolated from the throat swab of the same case.Conclusions COVID-19 cases have the possibility of co-infection with 2019-nCoV and HSV-1.

关 键 词：新型冠状病毒 单纯疱疹病毒I型 病毒分离 合并感染 

分 类 号：R563.1[医药卫生—呼吸系统]