新型冠状病毒肺炎确诊病例不同病程的新型冠状病毒核酸检测分析  

Detection of 2019-nCoV nucleic acid at different time of courses in confirmed COVID-19 cases

作　　者：薛泽润 尤海菲 吴瑞[1] 栾阳[1] 陈海龙[1] 杜全丽[1] 韩月雯 杨玉捷 邓杨妮 黄银娟[1] 李浩[1] 李凯欣 张磊乐 张鹏 马超锋[1] Xue Zerun;You Haifei;Wu Rui;Luan Yang;Chen Hailong;Du Quanli;Han Yuewen;Yang Yujie;Deng Yangni;Huang Yinjuan;Li Hao;Li Kaixin;Zhang Leile;Zhang Peng;Ma Chaofeng(Xi′an Municipal Center for Disease Control and Prevention,Xi′an 710054,China;Shaanxi Provincial Center for Disease Control and Prevention,Xi′an 710054,China)

机构地区：[1]西安市疾病预防控制中心,710054 [2]陕西省疾病预防控制中心,西安710054

出　　处：《中华实验和临床病毒学杂志》2021年第6期728-731,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：西安市新型冠状病毒肺炎疫情应急防治科技专项(20200002YX002-2,20200003YX003-1)。

摘　　要：目的分析新型冠状病毒肺炎(Coronavirus Disease 2019,COVID-19)确诊病例不同病程的核酸检测结果。方法选择COVID-19确诊病例,采集患者不同病程的鼻咽拭子标本,在患者治愈出院后进行随访,实时荧光RT-PCR方法检测病毒核酸。结果2020年1月23日至2月20日西安市累计报告120例COVID-19本土确诊病例,初检病毒核酸阳性率为70.83%(85/120)。COVID-19包括连续性核酸阳性和间歇性核酸阳性,患者在潜伏期即可检出核酸阳性,检出核酸阳性的最长发病时间是第26天。COVID-19确诊病例在发病初期病毒核酸阳性率较高,发病第6天的阳性率最高,为84.21%,核酸阳性率随时间推移逐渐降低,患者出院后第7天随访到105人,复阳率为2.86%(3/105)。结论COVID-19确诊病例在发病初期核酸阳性率较高,在潜伏期和发病后较长时间内可检出核酸阳性。Objective To explore the positive rates of 2019-nCoV nucleic acid at different time of courses of COVID-19.Methods Patients with confirmed COVID-19 were enrolled in this study.Nasal and throat swabs were collected from different courses of disease.Real-time RT-PCR was used to detect 2019-nCoV nucleic acid.Results From January 23 to February 20,2020,a total of 120 confirmed cases of COVID-19 were reported in Xi’an,and 85 cases(70.83%)were positive in first detection.The COVID-19 cases included consistently nucleic acid positive and intermittently nucleic acid positive patients.2019-nCoV nucleic acid could be detected in incubation period,and the longest observed duration of nucleic acid positive in this study was 26 days.The positive rate of 2019-nCoV nucleic acid was up to 84.21%on the 6th day,and the positive rate decreased as time passed during the course of COVID-19.Three patients(2.86%)were tested positive for 2019-nCoV nucleic acid again in nasal and throat swabs after discharge.Conclusions The positive rate of 2019-nCoV nucleic acid was higher in the early stage of disease.2019-nCoV nucleic acid can be detected in incubation period,and virus shedding may last for a long period.

关 键 词：新型冠状病毒肺炎 新型冠状病毒 确诊病例 核酸检测 

分 类 号：R563.1[医药卫生—呼吸系统]