支气管哮喘患儿血清硫化氢、嗜酸性粒细胞趋化因子水平与炎症因子及肺功能的关系研究  

Association of Serum H2S and Eotaxin Levels with Inflammatory Mediators and Pulmonary Function in Children with Bronchial Asthma

作　　者：豆吉娟[1] 朱炜杰[1] 周雪梅 贾红亮 曹亚船[1] 徐华 杨洋[2] DOU Ji-juan;ZHU Wei-jie;ZHOU Xue-mei;JIA Hong-liang;CAO Ya-chuan;XU Hua;YANG Yang(Department of Pediatrics,Affiliated Hospital of Jiangnan University,Wuxi,Jiangsu,214122,China;Department of Pediatrics,The Second Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu,210011,China)

机构地区：[1]江南大学附属医院儿科,江苏无锡214122 [2]南京医科大学第二附属医院儿科,江苏南京210011

出　　处：《现代生物医学进展》2021年第24期4751-4755,共5页Progress in Modern Biomedicine

基　　金：江苏省自然科学基金项目(BK20170948)。

摘　　要：目的:探讨支气管哮喘患儿血清硫化氢(H_(2)S)、嗜酸性粒细胞趋化因子(Eotaxin)水平与炎症因子及肺功能的关系。方法:选取我院收治的95例支气管哮喘患儿,按照《儿童支气管哮喘诊断及防治指南(2016年版)》将支气管哮喘患儿分为急性发作期组43例和缓解期组52例。检测血清中H_(2)S水平;检测Eotaxin、肿瘤坏死因子-α(TNF-α),白介素6(IL-6)和白介素13(IL-13)的水平;对患儿进行肺功能测定,采用Pearson检验分析血清H_(2)S、Eotaxin水平与血清炎症因子及肺功能之间的相关性。ROC曲线分析血清H_(2)S、Eotaxin对支气管哮喘患儿病情的预测价值。结果:急性发作期组患儿血清H_(2)S水平、第一秒用力呼气容积占预计值百分比(FEV_(1)%pred)、最大呼气流量占预计值百分比(PEF%pred)和FEV_(1)/用力肺活量(FVC)低于缓解期组患儿,Eotaxin、TNF-α、IL-6和IL-13水平高于缓解期组患儿(P<0.05);支气管哮喘患儿血清H_(2)S水平与TNF-α、IL-6和IL-13水平均呈负相关(P<0.05),与FEV_(1)%pred、FEV_(1)/FVC和PEF%pred均呈正相关(P<0.05);支气管哮喘患儿血清Eotaxin水平与TNF-α、IL-6和IL-13水平均呈正相关(P<0.05),与FEV_(1)%pred、FEV_(1)/FVC和PEF%pred均呈负相关(P<0.05)。血清H_(2)S预测支气管哮喘患儿病情的曲线下面积为0.854,灵敏度和特异度分别为77.89%和81.05%,Youden指数为0.5894;血清Eotaxin预测支气管哮喘患儿病情的曲线下面积为0.924,灵敏度和特异度分别为92.71%和84.38%,Youden指数为0.7709。结论:支气管哮喘急性发作期患儿与缓解期患儿相比,其血清H_(2)S水平较低,Eotaxin水平较高,血清H_(2)S和Eotaxin水平与机体炎症反应及肺功能密切相关,有助于辅助评估支气管哮喘患儿的病情。Objective:To investigate the Association of serum Hydrogen sulfide(H_(2)S)and Eosinophil chemokine(Eotaxin)Levels with inflammatory mediators and pulmonary Function in Children with Bronchial Asthma.Methods:95 children with bronchial asthma treated in our hospital were selected,and children with bronchial asthma were divided into acute exacerbation period group and remission period group according to the guidelines for the diagnosis and prevention of children’s asthma(2016 Edition).Ultraviolet-visible spectrophotometry was used to detect H_(2)S levels in serum;Eotaxin,Tumor necrosis factor-α(TNF-α),Interleukin 6(IL-6)and interleukin 13(IL-13)levels were detected by enzyme-linked immunosorbent assay.The pulmonary function of bronchial asthma children was measured by pulmonary function tester.The correlation between serum H_(2)S,eotaxin,serum inflammatory factors and pulmonary function was analyzed by Pearson test,ROC curve was used to analyze the predictive value of H_(2)S,Eotaxin to the condition of children with asthma.Results:Serum H_(2)S levels,percentage of forced expiratory volume in the first second to the predicted value(FEV_(1)%pred),percentage of the maximum expiratory flow to the predicted value(PEF%pred),and FEV_(1)/Forced vital capacity(FVC)in children with bronchial asthma during acute exacerbation period were lower than those during asthma remission period,and serum levels of Eotaxin,TNF-α,IL-6,and IL-13 were higher in children with asthma during asthma remission period(P<0.05).Serum H_(2)S levels in children with bronchial asthma were negatively correlated with levels of TNF-α,IL-6 and IL-13(P<0.05),and positively correlated with FEV_(1)%pred,FEV_(1)/FVC and PEF%pred(P<0.05).Serum Eotaxin levels in children with bronchial asthma were positively correlated with the levels of TNF-α,IL-6and IL-13,and negatively correlated with FEV_(1)%pred,FEV_(1)/FVC and PEF%pred(P<0.05).The area under the curve of serum H_(2)S to predict the condition of children with bronchial asthma was 0.854,the sensitivit

关 键 词：支气管哮喘 患儿 硫化氢 嗜酸性粒细胞趋化因子 炎症因子 肺功能 

分 类 号：R562.25[医药卫生—呼吸系统]