植物天然产物途径创建  

Biopathway construction of plant natural products

作　　者：刘秀玉 罗凌龙 马莹[1] 卜俊玲 胡志敏 孙术富 崔光红[1] 唐金富[1] 郭娟 黄璐琦[1] LIU Xiu-yu;LUO Ling-long;MAYing;BU Jun-ling;HU Zhi-min;SUN Shu-fu;CUI Guang-hong;TANG Jin-fu;GUO Juan;HUANG Lu-qi(State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Pharmaceutical Sciences,Henan University of Chinese Medicine,Zhengzhou 450046,China;School of Pharmaceutical Sciences,Anhui University of Chinese Medicine,Hefei 230071,China)

机构地区：[1]中国中医科学院中药资源中心,道地药材国家重点实验室,北京100700 [2]河南中医药大学药学院,河南郑州450046 [3]安徽中医药大学药学院,安徽合肥230071

出　　处：《药学学报》2021年第12期3285-3299,共15页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划资助项目(2020YFA0908000);国家自然科学基金资助项目(81822046,31961133007,82011530137);中央本级重大增减支项目“名贵中药资源可持续利用能力建设”资助项目(2060302);中央级公益性科研院所基本科研业务费专项资金资助项目(ZZ13-YQ-083-A1);国家中医药管理局中医药创新团队及人才支持计划资助项目(ZYYCXTD-D-202005)。

摘　　要：植物天然产物是创新药物的重要源泉,主要从植物中分离提取获得,但由于含量低、结构类似物多、生产成本高等因素限制了对植物天然产物的深入开发利用。植物天然产物途径创建通过生物合成途径解析、代谢途径重构和优化,能够实现植物天然产物的异源合成,为其提供高效稳定的绿色获取方式。随着国际上具有影响力的研究工作的报道,基于我国丰富的药用植物资源和深厚的中医药研究经验,植物天然产物尤其是具有明确药理活性的中药活性成分的生物合成途径解析在国内广受关注,并取得了丰硕研究成果。本文综述了植物天然产物生物合成及其合成生物学生产的研究进展和思路,对近年新方法新技术的应用进行总结和讨论,为植物天然产物途径创建提供参考。Plant natural products(PNPs)are important sources of innovative drugs.They are mainly obtained by isolation or extraction from plants.Low content and with structural analogues in plants result in high production cost,which restricts the research and application of PNPs.While biopathway construction by synthetic biology provides an alternative for production of PNPs.By biosynthetic pathway analysis of PNPs and reconstructing the biopathway in microorganisms,we can produce PNPs in cell factories efficiently.Recently,several predominantly international reports about biosynthesis of PNPs and its synthetic biology production,triggered the researches of PNPs.Abundant traditional Chinese medicine resources and profound cultural heritage of Chinese medicine make biosynthesis pathway analysis of PNPs to be a research hotspot.And some of the studies have achieved significant progress.Here,recent progress in the biosynthesis of plant natural products and its synthetic biology was reviewed.In particular,the application of new methods and technologies in recent years were summarized and discussed.This will provide reference for the biopathway construction of plant natural products.

关 键 词：植物天然产物 结构修饰酶 生物合成途径 合成生物学 

分 类 号：R931[医药卫生—生药学]