中药现代化研究的崭新模式:单细胞药理学  

A new research paradigm in modernization of traditional Chinese medicine:single cell pharmacology

作　　者：陈嘉鋆 郭秋岩 徐承超 王继刚 CHEN Jia-yun;GUO Qiu-yan;XU Cheng-chao;WANG Ji-gang(Artemisinin Research Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院青蒿素研究中心,中药研究所,北京100700

出　　处：《药学学报》2021年第12期3300-3312,共13页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82074098,81841001,82104480);国家重点研发计划专项资助项目(2020YFA0908000,2020YFA0908004,2020YFA0908001);北京市科学技术协会青年人才托举工程2021项目;中央级公益性科研院所基本科研业务费专项资金资助项目(ZZ13-ZD-07,ZZ14-YQ-050,ZZ14-YQ-051,ZZ14-YQ-060,ZZ14-ND-010,ZZ15-ND-10,CI2021A05101)。

摘　　要：单细胞组学技术能够在单细胞分辨率下以高通量、无偏倚的方式获取单个细胞的基因组、转录组、蛋白质组等组学信息,从而全面准确地表征细胞功能状态、揭示细胞间异质性以及反映药物治疗后的效应差异。该技术广泛应用于现代药物的药物筛选、疗效评价和药理研究等。在中药研究领域,单细胞组学技术在药物有效成分筛选、作用靶标鉴定以及作用机制阐明等方面具有巨大的应用潜力与发展空间。本文简要介绍以单细胞转录组测序为代表的单细胞组学技术,综述其在现代药物研究领域的应用。在此基础上展望单细胞组学技术应用于中药药理研究的崭新模式——单细胞药理学,系统阐述其在中药药理中的研究模式以及在促进中药现代化中的应用前景。Single cell"omics"technology enables the capture of genome,transcriptome,proteome and other omics information in a high-throughput and unbiased manner at single-cell resolution,allowing the characterization of the functional state of individual cells to reveal their heterogeneity and differential responses to drug treatment.This technology has wide application in pharmacological research,facilitating drug screening,efficacy evaluation,and mechanistic studies.We envision that,in the field of traditional Chinese medicine(TCM),single cell omics technology can be applied in the identification of active ingredients and drug targets,and elucidation of drug mechanism of action.In this article,we briefly introduce the single cell omics technology-particularly single cell transcriptome sequencing,and review its application in the field of modern drug research.Based on that,we propose the concept of"single cell pharmacology"and articulate how it can be applied to transform the pharmacological research of TCM and promote TCM modernization.

关 键 词：单细胞药理学 单细胞组学技术 中药现代化 

分 类 号：R931[医药卫生—生药学]