植物miRNA研究方法与药用植物miRNA研究进展  

Research methods of miRNA in plants and research progress of miRNA in medicinal plants

作　　者：郑小宇 陈敏 李航 赵淑娟 ZHENG Xiao-yu;CHEN Min;LI Hang;ZHAO Shu-juan(Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,The SATCM Key Laboratory for New Resources&Quality Evaluation of Chinese Medicine,The MOE Key Laboratory for Standardization of Chinese Medicines,Shanghai Key Laboratory of Compound Chinese Medicines,Shanghai 201203,China;Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Xingling Science and Technology Pharmaceutical Co.,Ltd.,Shanghai 201703,China)

机构地区：[1]上海中医药大学中药研究所,中药新资源与品质评价国家中医药管理局重点研究室,教育部中药标准化研究中心,上海市复方中药重点研究室,上海201203 [2]上海中医药大学教学实验中心,上海201203 [3]上海上药杏灵科技药业股份有限公司,上海201703

出　　处：《药学学报》2021年第12期3460-3472,共13页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金面上项目(81673540);上海市自然科学基金资助课题(16ZR1434100)。

摘　　要：miRNA(microRNA)是一类长度约为21nt的内源性非编码单链小RNA,在植物生长发育、信号转导、环境胁迫响应和次生代谢产物形成等方面发挥着重要的调控作用。近年来,多种药用植物中鉴定出了大量miRNA,并初步研究了这些miRNA对药用植物的调控作用。药用植物的活性成分大多是其次生代谢产物,研究miRNA对药用植物次生代谢产物形成的调控作用具有十分重要的意义。本文综述了植物miRNA的一般研究方法与药用植物miRNA的研究进展及其对具有药用活性的代谢产物形成的调控作用,并对药用植物miRNA发展趋势做了展望,为药用植物的研究开拓新思路。microRNA(miRNA)is a class of endogenous~21 nt non-coding single-strand small RNAs which play important roles in plant growth and development,signal transduction,stress response,and secondary metabolism.In recent years,a large number of miRNAs have been identified in various medicinal plants,and the regulatory effects of these miRNAs have been preliminarily studied.In medicinal plants,most of the active components are secondary metabolites,so it is of great significance to study the regulatory effects of miRNA on the formation of secondary metabolites.In this paper,the general research methods of plant miRNA and the research progress of medicinal plant miRNA and their regulatory effects on the formation of bioactive metabolites were reviewed,and the future direction of medicinal plant miRNA was prospected,so as to provide reference for the future research of medicinal plants.

关 键 词：药用植物 MICRORNA 研究方法 次生代谢 

分 类 号：R931[医药卫生—生药学]