粉防己生物碱成分研究  

Study on alkaloid constituents of Stephania tetrandra S.Moore

作　　者：何达海 刘军[2] 方东梅 王晓玲 李丽梅 HE Da-hai;LIU Jun;FANG Dong-mei;WANG Xiao-ling;LI Li-mei(College of Pharmacy,Southwest Minzu University,Chengdu 610041,China;College of Chemistry and Environment,Southwest Minzu University,Chengdu 610041,China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China)

机构地区：[1]西南民族大学药学院,四川成都610041 [2]西南民族大学化学与环境学院,四川成都610041 [3]中国科学院成都生物研究所,四川成都610041

出　　处：《药学学报》2021年第12期3503-3510,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(21776231);中央高校基本科研业务费专项资金项目(2020NTD05)。

摘　　要：运用硅胶、反相硅胶、凝胶柱色谱,以及半制备高效液相色谱技术,从粉防己70%乙醇水提取物中分离得到15个生物碱类化合物,通过质谱、核磁共振波谱,以及单晶衍射数据,分别鉴定为tetrandraside A(1)、(Z)-N-formylnornuciferin(2)、(E)-N-formyl-nornuciferin(3)、salutaridine(4)、salutaridine N-oxide(5)、(E)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxy-3-methoxyphenyl)ethyl]-2 propenamide(6)、dauriporphine(7)、sinomenine(8)、liriodenine(9)、α-magnoflorine(10)、(1S)-4’-β-glucosylcoclaurine(11)、tetrandrine(12)、fangchinoline(13)、tetrandrine 2’-β-oxide(14)和tetrandrine 2’-α-oxide(15)。其中,化合物1为新的生物碱苷类化合物,化合物2~11为首次从粉防己中分离得到。这些化合物对肺癌耐药细胞株H1299均显示了很好的细胞毒活性,化合物9的活性最佳,其IC_(50)为5.38μmol·L^(-1)。Fifteen alkaloid compounds were isolated from the 70%aqueous alcohol extract of Stephania tetrandra S.Moore by silica gel,reversed phase silica gel,Sephadex LH-20 column chromatography and semi-preparative high performance liquid chromatography.They were identified as tetrandraside A(1),(Z)-N-formyl-nornuciferin(2),(E)-N-formyl-nornuciferin(3),salutaridine(4),salutaridine N-oxide(5),(E)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxy-3-methoxyphenyl)ethyl]-2 propenamide(6),dauriporphine(7),sinomenine(8),liriodenine(9),α-magnoflorine(10),(1 S)-4’-β-glucosylcoclaurine(11),tetrandrine(12),fangchinoline(13),tetrandrine 2’-β-oxide(14),and tetrandrine 2’-α-oxide(15),respectively,by MS,NMR and single crystal diffraction.Among them,compound 1 is a new alkaloid glycoside.Compounds 2-11 were obtained from this plant for the first time.These compounds showed obvious cytotoxic activity against drug-resistant lung cancer cell line H1299,and compound 9 had the best activity,with an IC_(50) of 5.38μmol·L^(-1).

关 键 词：粉防己 生物碱 单晶衍射 tetrandraside A 

分 类 号：R284[医药卫生—中药学]