生理性低氧条件下脐带和经血来源间充质干细胞的生物学特性  

Biological characteristics of umbilical cord and menstrual blood-derived mesenchymal stem cells under physiological hypoxia

作　　者：莫芸芳 王泽剑[1] 齐念民 陈彦田[1] Mo Yunfang;Wang Zejian;Qi Nianmin;Chen Yantian(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China;Shanghai Likun Bio-Technology Co.,Ltd.,Shanghai 201400,China)

机构地区：[1]上海交通大学药学院,上海市200240 [2]上海丽坤生物科技有限公司,上海市201400

出　　处：《中国组织工程研究》2022年第30期4819-4825,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81871122),项目负责人:王泽剑。

摘　　要：背景:间充质干细胞具有治疗糖尿病、移植物抗宿主病、卵巢早衰等多种疾病的潜能,但是传统培养方法存在着细胞增长缓慢、容易衰老等问题。生理性低氧有望改善间充质干细胞的生物学性能,因而有可能作为优化的培养方法。目的:探究生理性低氧对脐带间充质干细胞和经血源性间充质干细胞生物学特性的影响。方法:将脐带间充质干细胞和经血源性间充质干细胞分别在21%、5%氧体积分数条件下培养,测定细胞的表面标志物、贴壁效率、增殖能力、细胞凋亡、细胞衰老、葡萄糖乳酸代谢和细胞分化等指标。结果与结论:①低氧可以促进两种来源间充质干细胞的增殖,低氧条件下两种来源间充质干细胞的群体倍增时间均减小(脐带:P<0.001;经血:P<0.05);②低氧条件下,两种细胞乳酸代谢增强(脐带:P<0.05;经血:P<0.01),脐带间充质干细胞葡萄糖代谢增多(P<0.001);③低氧抑制高代数经血源性间充质干细胞的衰老;④低氧提高两种来源间充质干细胞的贴壁效率(P<0.01);⑤低氧对两种来源间充质干细胞的干性没有影响;⑥结果提示,生理性低氧可以改善脐带和经血来源间充质干细胞的增殖能力和贴壁效率,可以对衰老细胞起到抗衰老作用,同时对表面标志物无显著性影响。此外,低氧对不同来源间充质干细胞在分化上的影响存在着差异。BACKGROUND:Mesenchymal stem cells have the potential to treat various diseases such as diabetes,graft-versus-host disease,and premature ovarian failure.However,traditional culture methods have problems such as slow growth rate and senescence.Physiological hypoxia is expected to improve the biological characteristics of mesenchymal stem cells,which can optimize the culture method.OBJECTIVE:To explore the effects of physiological hypoxia on the biological characteristics of umbilical cord mesenchymal stem cells and menstrual bloodderived mesenchymal stem cells.METHODS:Umbilical cord mesenchymal stem cells and menstrual blood-derived mesenchymal stem cells were cultured under 21%and 5%oxygen concentrations.Cell surface markers,colony formation,proliferation,apoptosis,senescence,glucose and lactate metabolism and differentiation were detected.RESULTS AND CONCLUSION:(1)Hypoxia can promote the proliferation of umbilical cord mesenchymal stem cells and menstrual blood-derived mesenchymal stem cells,and the population doubling time of the two kinds of mesenchymal stem cells was reduced(umbilical cord:P<0.001;menstrual blood:P<0.05).(2)The lactate metabolism of the two kinds of stem cells was enhanced(umbilical cord:P<0.05;menstrual blood:P<0.01)under hypoxia and glucose metabolism of umbilical cord mesenchymal stem cells was enhanced(P<0.001).(3)Hypoxia improved senescence of menstrual blood-derived mesenchymal stem cells under high generation.(4)Hypoxia promoted the adhesion efficiency of umbilical cord mesenchymal stem cells and menstrual blood-derived mesenchymal stem cells(P<0.01).(5)Hypoxia had no effect on the stemness.(6)The results indicated that physiological hypoxia improved the proliferation and attachment efficiency of umbilical cord mesenchymal stem cells and menstrual blood derived mesenchymal stem cells and had an anti-aging effect on senescent cells,simultaneously had no effects on surface markers.In addition,hypoxia displayed different effects on the differentiation of mesenchymal stem cells derived from

关 键 词：干细胞 间充质干细胞 生理性低氧 生物学特性 增殖 衰老 代谢 分化 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]