长枝木霉T6与阿维菌素复配对禾谷孢囊线虫的室内毒杀效果  

Indoor Toxic Effect Determination of Trichoderma longibrachiatum T6 and Avermectin Combination against Cereal Cyst Nematode

作　　者：吉宝丽 毛维兴 张树武 徐秉良 JI Baoli;MAO Weixin;ZHANG Shuwu;XU Bingliang(College of Plant Protection,Gansu Agricultural University,Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《西北农业学报》2022年第2期233-239,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陇原青年创新创业人才项目(LYRC2019-35);国家自然基金(31860526);甘肃省杰出青年基金(18JR3RA161);甘肃农业大学人才专项经费(2017RCZX-07);甘肃农业大学学科建设基金(GAU-XKJS-2018-147);甘肃农业大学“伏羲杰出人才培育计划”(Gaufx-03J03)。

摘　　要：旨在采用室内毒力测定法测定长枝木霉与阿维菌素复配对禾谷孢囊线虫2龄幼虫的毒杀效果。试验结果表明,单一长枝木霉T6菌株分生孢子悬浮液和阿维菌素对禾谷孢囊线虫2龄幼虫均表现出较好的毒杀效果,且不同浓度之间存在显著差异,随着处理浓度和时间的增加,毒杀作用增强。经2.0×107 cfu·mL^(-1)的木霉分生孢子悬浮液和0.5μg·mL^(-1)的阿维菌素溶液处理2龄幼虫48 h后,线虫死亡率分别达到71.43%和60.28%;当长枝木霉T6与0.5μg·mL^(-1)的阿维菌素溶液按1∶1比例复配时,其对2龄幼虫的毒杀活性最佳,处理48 h后线虫死亡率高达96.30%,其毒杀作用显著优于单一长枝木霉T6和单一阿维菌素溶液。Indoor toxicity determination method was used to determine the toxic effect of the Trichoderma longibrachiatum,avermectin and complex against second stage juveniles of cereal cyst nematode(Heterodera avenae).The results showed that the conidia suspension of T.longibrachiatum T6 and avermectin solution had strong toxic effect on the second stage juveniles,and significant difference existed among the treatments with different concentrations of avermectin.In addition,the mortality of nematodes increased with increase of the concentration and extension of the time.The mortality of nematodes treated with the concentrations of 2.0×107 cfu·mL^(-1) T.longibrachiatum and 0.5μg·mL^(-1) avermectin were 71.43%and 60.28%after 48 h.The combination of T.longibrachiatum and 0.5μg·mL^(-1) avermectin solution presented the highest toxic effecton the nematodes at the ratio of 1∶1 after inoculation.The mortality rate of nematode was 96.30%after 48 hours,which was significantly higher than the single biocontrol preparation.The results provide an important theoretical basis for obtaining an efficient compound biopesticide.

关 键 词：长枝木霉 禾谷孢囊线虫 阿维菌素 复配 毒杀作用 

分 类 号：S432.45[农业科学—植物病理学]