电针刺激对大鼠脊髓损伤部位神经胶质纤维酸性蛋白的影响  

Effect of electroacupuncture on glial fibrillary acidic protein expression at the injured site in a rat model of spinal cord injury

作　　者：唐福宇 周宾宾[2] 魏卫兵 张鸿升 Tang Fuyu;Zhou Binbin;Wei Weibing;Zhang Hongsheng(Liuzhou Traditional Chinese Medicine Hospital(Liuzhou Zhuang Medical Hospital),Liuzhou 545000,Guangxi Zhuang Autonomous Region,China;the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 543000,Guangxi Zhuang Autonomous Region,China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 543000,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]柳州市中医医院(柳州市壮医医院),广西壮族自治区柳州市545000 [2]广西中医药大学第一附属医院,广西壮族自治区南宁市543000 [3]广西中医药大学附属瑞康医院,广西壮族自治区南宁市543000

出　　处：《中国组织工程研究》2022年第26期4113-4117,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81660814):电针通过Nogo-A/NgR与NGF/TrkA的crostalk调节SCI后神经可塑性的机制研究,项目负责人:周宾宾;广西自然科学基金(2019JJA140167),项目负责人:唐福宇;柳州市科技计划项目(2019BE10602):提针治疗背肌筋膜炎的临床研究及机制探讨,项目负责人:唐福宇。

摘　　要：背景:神经胶质纤维酸性蛋白是一个中间丝过渡表达蛋白,其表达水平是最常用的星形胶质细胞的特异性标志蛋白。中医治疗脊髓损伤历史悠久,大量研究表明针刺“督脉”“足阳明胃经”对脊髓损伤的神经再生修复具有一定作用。目的:观察电针刺激后脊髓损伤大鼠损伤部位神经胶质纤维酸性蛋白表达的变化,进一步探讨电针刺激对脊髓损伤大鼠神经再生的影响。方法:将120只SD大鼠随机分为5组,即督脉电针组、胃经电针组、混合电针组、模型对照组和假手术组,每组24只,除假手术组不进行脊髓切断外,其他各组均制备T_(10)脊髓半横断损伤模型。每个组再随机分为治疗后3,7,14,21 d组,每亚组6只。假手术组及模型对照组不进行干预,其他3个电针组进行相应电针干预。以免疫组化法检测损伤局部神经胶质纤维酸性蛋白阳性细胞的表达;PCR、Western blot检测神经胶质纤维酸性蛋白mRNA及蛋白的表达情况。结果与结论:①模型对照组损伤后神经胶质纤维酸性蛋白的表达水平呈先增高后降低的趋势,与未损伤的假手术组相比差异有显著性意义(P<0.05);②督脉电针组、胃经电针组与模型对照组相比,神经胶质纤维酸性蛋白表达水平明显降低(P<0.05);③混合电针组与督脉电针组、胃经电针组相比神经胶质纤维酸性蛋白表达水平降低,但差异无显著性意义(P>0.05);④提示电针刺激可降低损伤局部神经胶质纤维酸性蛋白的表达,减少损伤局部星形胶质细胞的表达,从而抑制胶质瘢痕的形成。BACKGROUND:Glial fibrillary acidic protein is an intermediate filament overexpression protein,which is the most commonly used specific marker protein for astrocytes.Traditional Chinese medicine has a long history of treating spinal cord injury.Numerous studies have shown that acupuncture at Du Meridian and Stomach Meridian of Foot-Yangming has a certain therapeutic effect on nerve regeneration and repair after spinal cord injury.OBJECTIVE:To observe the changes in the expression of glial fibrillary acidic protein in the injured part of spinal cord injury rats after electroacupuncture stimulation,and to further investigate the effect of electroacupuncture on nerve regeneration in rats with spinal cord injury.METHODS:A total of 120 Sprague-Dawley rats were randomly divided into 5 groups(n=24 per groups):a Du Meridian electroacupuncture group,a Stomach Meridian of Foot-Yangming electroacupuncture group,a mixed electroacupuncture group,a model control group,and a sham operation group.T10 spinal cord hemisection models were prepared in all the groups except for the sham operation group.After modeling,each group was randomly subdivided into four subgroups(n=6 per group):a 3-day group,a 7-day group,a 14-day group,and a 21-day group.The sham operation group and the model control group were not given an intervention,and the three electroacupuncture groups were given a corresponding electroacupuncture intervention.Immunohistochemical staining was used to detect the expression of glial fibrillary acidic protein positive cells in the injured area.PCR and western blot were used to detect the mRNA and protein expression of glial fibrillary acidic protein.RESULTS AND CONCLUSION:Compared with the sham operation group,the expression of glial fibrillary acidic protein after injury was increased first and then decreased significantly in the model control group(P<0.05).Compared with the model control group,the expression of glial fibrillary acidic protein was significantly reduced in the Du Meridian electroacupuncture group and the

关 键 词：电针刺激 脊髓损伤 胶质纤维酸性蛋白 督脉 胃经 神经再生 

分 类 号：R459.9[医药卫生—治疗学] R245[医药卫生—临床医学]