巴氏芽孢杆菌生物修复重金属污染溶液试验研究  

Experimental investigation on bioremediation of heavy metal contaminated solution by Sporosarcina pasteurii

作　　者：黄小松 章荣军[1] 崔明娟[1] 郑俊杰[1] HUANG Xiaosong;ZHANG Rongjun;CUI Mingjuan;ZHENG Junjie(Institute of Geotechnical and Underground Engineering,Huazhong University of Science and Technology,Wuhan 430074,P.R.China)

机构地区：[1]华中科技大学岩土与地下工程研究所,武汉430074

出　　处：《土木与环境工程学报（中英文）》2022年第3期160-167,共8页Journal of Civil and Environmental Engineering

基　　金：国家重点研发计划(2018YFC1802302)。

摘　　要：垃圾填埋场中重金属污染问题严峻,亟待高效的修复技术予以处理。近年来,MICP技术修复重金属污染土体/水体的潜力被广泛探究。在众多土著微生物中,巴氏芽孢杆菌脲酶活性和重金属耐受性更佳,但少有研究探究巴氏芽孢杆菌对高浓度重金属的固定/去除潜力,且仅利用MICP理论难以解释巴氏芽孢杆菌对不同重金属的固定/去除机理,此外,常见生物修复工艺的效率有待进一步提高。为避免填埋场中腐殖土和渗滤液物化特性的影响,以重金属污染溶液为研究对象,采用简单拌和法开展生物修复试验;基于MICP和化学平衡理论,利用Visual MINTEQ软件对生物修复重金属污染溶液的过程进行简化模拟。结果表明:高浓度范围内,巴氏芽孢杆菌可通过生物矿化和生物富集作用有效去除溶液中的Cd、Cr(Ⅲ)和Zn等重金属;此外,简单拌和法操作简便,生物修复效率高,具有工程应用的潜力。It is urgent to deal with the severe problem of heavy metal pollution in landfills using efficient remediation technology.In recent years,the potential of MICP technology to remedy heavy metals contaminated soil/water has been extensively explored.Sporosarcina pasteurii shows better urease activity and heavy metal tolerance among the indigenous microorganisms.However,few researches have explored the immobilization/removal potential of S.pasteurii to heavy metals in high concentration.Then,it is difficult to explain the immobilization/removal mechanism of S.pasteurii to different healy metals using only MICP theory.In addition,the efficiency of commonly used bioremediation processes needs to be further improved.To avoid the impact of humus and leachate in the landfill,this study took heavy metal contaminated solutions as the research object and used simple mixing methods to carry out bioremediation experiments.Based on the MICP and chemical equilibrium theory,the Visual MINTEQ software was used to simplify and simulate the bioremediation process of heary metal contaminated solutions.The results show that in the high concentration range,S.pasteurii can efficiently remove heavy metal such as Cd,Cr(Ⅲ)and Zn through biomineralization and bioaccumulation.In addition,the simple mixing method is easy to operate,the bioremediation efficiency is high,and it has the potential for engineering applications.

关 键 词：巴氏芽孢杆菌 生物修复 重金属 简单拌和法 

分 类 号：X505[环境科学与工程—环境工程]