生物气溶胶监测仪生物计数评价装置的搭建和方法研究  

Device Construction and Method Research of Biological Counting Evaluation of Bioaerosol Detectors

作　　者：田莹 张国城 吴丹 刘佳琪 沈上圯 潘一廷 李晶晶 TIAN Ying;ZHANG Guo-cheng;WU Dan;LIU Jia-qi;SHEN Shang-yi;PAN Yi-ting;LI Jing-jing(Beijing Institute of Metrology,Beijing 100029,China;College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China)

机构地区：[1]北京市计量检测科学研究院,北京100029 [2]北京大学环境科学与工程学院,北京100871

出　　处：《计量学报》2021年第12期1687-1692,共6页Acta Metrologica Sinica

基　　金：2021年度国家市场监管总局科技计划项目(2021MK003)。

摘　　要：生物气溶胶监测仪基于生物分子激光诱导荧光的原理监测生物性颗粒物的存在和数量,其发展对公共卫生安全以及国防安全的早期预警具有重要意义。通过设计并搭建一套生物气溶胶监测仪的校准装置,雾化菌液后,利用菌落计数法评价生物气溶胶监测仪的关键技术指标,研究了菌种,菌液浓度,荧光强度等因素对监测结果的影响。结果表明:搭建的装置产生的生物气溶胶足够均匀稳定,通过采样菌落计数法对气溶胶浓度进行定值,初步实现对生物气溶胶监测仪的校准。The bioaerosol detector monitors the existence and quantity of biological particles based on the principle of laser-induced fluorescence, and its development is of great significance to the early warning of public health and national defense security. Through designing and constructing a calibration device, the colony counting method is used to evaluate the key technical indicators of the bioaerosol detector after nebulizing the bacteria liquid, and the effects of bacterial species, bacterial solution concentration and fluorescence intensity on the monitoring results are studied.The results show that bioaerosol produced in the calibration device is sufficiently uniform and stable. The aerosol concentration is determined by the colony counting method, and the calibration of the bioaerosol detectors is initially realized.

关 键 词：计量学 生物气溶胶监测仪 菌落计数 荧光强度 校准 

分 类 号：TB99[一般工业技术—计量学]