淡色库蚊羧酸酯酶基因高表达与溴氰菊酯抗药性相关性研究  

CARBOXYLESTERASE GENE OVEREXPRESSION AND DELTAMETHRIN RESISTANCE IN CULEX PIPIENS PALLENS

作　　者：吕园 邱樊 张红波 徐敬薇 沈波[2] LYU Yuan;QIU Fan;ZHANG Hong-Bo;XU Jing-Wei;SHEN Bo(Department of Nuclear Medicine,Nanjing First Hospital,Nanjing Hospital Affiliated to Nanjing Medical University,Nanjing 210006,Jiangsu,China;Department of Pathogen Biology,Nanjing Medical University,Nanjing 210000,Jiangsu,China)

机构地区：[1]南京市第一医院,南京医科大学附属南京医院核医学科,江苏南京210006 [2]南京医科大学病原生物学系,江苏南京210000

出　　处：《寄生虫与医学昆虫学报》2021年第4期231-236,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金项目(11805104)。

摘　　要：采用荧光定量PCR法,检测羧酸酯酶基因(CPIJ002786-RA)在淡色库蚊实验室敏感品系、抗性选育品系及4个现场种群(山东商河、山东孤岛、山东惠民、江苏江心洲)敏感株和抗性株中的相对表达水平。结果显示,幼虫浸渍法检测实验室敏感品系LC_(50)(半数致死浓度)0.03 mg/L,抗性选育品系LC_(50)为2.00 mg/L。WHO成蚊接触筒法(0.05%溴氰菊酯药膜)区分现场种群敏感株和抗性株,平均死亡率在48%~72%之间,敏感株平均击倒时间在32~52 min之间。淡色库蚊羧酸酯酶基因(CPIJ002786-RA)表达水平,实验室抗性选育品系是敏感品系的2.77倍(P<0.01),山东商河、孤岛、惠民3个现场种群抗性株分别是敏感株的3.63(P<0.05)、5.67(P<0.05)和2.75倍(P<0.01),江苏江心洲现场种群抗性株和敏感株无显著差异(P>0.05)。淡色库蚊羧酸酯酶基因(CPIJ002786-RA)高表达可能与溴氰菊酯抗药性密切相关,具有作为蚊抗药性现场检测靶标的潜在应用价值。Fluorescence quantitative PCR method was used to detect the relative expression levels of carboxylesterase gene(CPIJ002786-RA)in sensitive and resistant strains of Culex pipiens pallens of both laboratory population(LC_(50)=0.03 mg/L and 2.00 mg/L by larval bioassay),and sensitive and resistant strains(differentiated by WHO susceptibility tube bioassay)in 4 field populations(Shanghe/Gudao/Huimin of Shandong Province,Jiangxinzhou of Jiangsu Province).The expression level of carboxylesterase(CPIJ002786-RA)was 2.77-fold higher in resistant strains than in susceptible strains of laboratory populations(P<0.01),and were 3.65-,5.67-and 2.75-fold higher in three resistant strains of field populations(Shanghe,Gudao and Huimin)than in susceptible strains(P<0.05).However,the expression level of carboxylesterase was no significant difference between resistant and sensitive strains(P>0.05)in Jiangxinzhou field population.The over expression of carboxylesterase gene(CPIJ002786-RA)in Cx.pipiens pallens may be closely related to the deltamethrin resistance,which means a potential application value as an index gene in field resistance detection.

关 键 词：淡色库蚊 溴氰菊酯 羧酸酯酶 荧光定量PCR 现场抗药性检测 

分 类 号：R384.1[医药卫生—医学寄生虫学]