特早熟甘蓝型春油菜恢复系核心种质构建  

Core Collection Construction of Extra-Early Restorer Lines in Spring Brassica napus L.

作　　者：林晓阳 杜德志[1] 柳海东[1] 李钧[1] Lin Xiaoyang;Du Dezhi;Liu Haidong;Li Jun(Academy of Agricultural and Forestry Sciences of Qinghai University/Key Laboratory of Spring Rapeseed Genetic Improvement/The Qinghai Research Branch of the National Rapeseed Genetic Improvement Center/Spring Rape Scientific Observation Experimental Station of Ministry of Agriculture and Rural Affairs/Qinghai Research and Development Center for Spring Rapeseed/Qinghai Province Spring Rape Engineering Technology Research Center,Xining 810016,Qinghai,China)

机构地区：[1]青海大学农林科学院/春油菜遗传改良重点实验室/国家油菜改良中心青海分中心/农业农村部春油菜科学观测实验站/青海省春油菜研究开发中心/青海省春油菜工程技术研究中心,青海西宁810016

出　　处：《作物杂志》2022年第1期31-37,共7页Crops

基　　金：国家油菜产业技术体系(CARS-12);高产优质抗倒新品种青杂15号产业化技术研究与示范(2020-NK-116);青海省春油菜遗传改良重点实验室(2020-ZJ-Y10)。

摘　　要：通过对特早熟甘蓝型春油菜恢复系种质资源进行评价和利用,降低育种工作中恢复系与不育系选配杂交组合的工作量,从而更好地为杂交种选育服务。通过对97份波里马雄性不育系的恢复系进行简化基因组(SLAFseq)测序,开发SNP标记,进而分析群体遗传关系,构建核心种质群体。结果显示,共获得527872个SLAF标签,842248个有效SNP;利用有效SNP对97份种质资源进行聚类分析,可将其分为5类;利用Core Hunter构建核心种质,构建了C30核心种质29份,C40核心种质38份,C30和C40核心种质平均等位基因覆盖度分别为99.89%和99.96%,这些材料能够代表整个资源的变异程度,达到了构建核心种质的要求。In order to better evaluate and utilize the germplasm resources of extra-early restorer lines of spring Brassica napus L.,to reduce the workload of selecting hybrid combinations of restorer lines and sterile lines in breeding work,and service for hybrids selection and breeding,this paper analyzes the population genetic relationships of Pol cytoplasmic male sterility by specific-locus amplified sequencing(SLAF-seq)of 97 restorer lines and developing SNP markers.Core Hunter software was used to construct a core collection.The results showed that a total of 527872 SLAF tags and 842248 effective SNPs were obtained.Through the effective SNP cluster analysis of 97 lines,these materials were divided into five categories.Two core collections of C30(29 lines)and C40(38 lines)were constructed.The coverage of alleles of C30 core collection was 99.89%,while that of C40 was 99.96%.These materials could represent the degree of variation of the entire resources,which met the requirements for constructing core collection.

关 键 词：特早熟 甘蓝型油菜 核心种质 波里马雄性不育 

分 类 号：S565.4[农业科学—作物学]