基于叶绿素荧光的小麦越冬期冻害评价  

Evaluation of Wheat Freezing Damage during Overwintering Period Based on Chlorophyll Fluorescence

作　　者：杨程[1] 杜思梦 张德奇[1] 时艳华[1] 李向东[1] 邵运辉[1] 方保停[1] 王汉芳[1] Yang Cheng;Du Simeng;Zhang Deqi;Shi Yanhua;Li Xiangdong;Shao Yunhui;Fang Baoting;Wang Hanfang(Wheat Research Institute,Henan Academy of Agricultural Sciences/National Laboratory of Wheat Engineering/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-Huai Region,Ministry of Agriculture and Rural Affairs/Scientific Observing and Experimental Station of Crop Cultivation in Central Plains,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory of Wheat Biology,Zhengzhou 450000,Henan,China)

机构地区：[1]河南省农业科学院小麦研究所/小麦国家工程实验室/农业农村部黄淮中部小麦生物学与遗传育种重点实验室/农业农村部中原地区作物栽培科学观测实验站/河南省小麦生物学重点实验室,河南郑州450000

出　　处：《作物杂志》2022年第1期154-160,共7页Crops

基　　金：河南省农业科学院优秀青年科技基金;国家自然科学基金(31601242)。

摘　　要：低温冻害是小麦生产中常见的自然灾害,目前小麦低温抗性的无损评价方法还非常有限。快速叶绿素荧光诱导动力学曲线包含了丰富的光合作用相关信息,是研究植物逆境胁迫的常用方法。通过筛选合适的叶绿素荧光参数来评价小麦的抗寒性,为小麦抗寒机理、越冬期管理和抗寒育种提供技术支持。通过调查越冬期冻害发生年份34个常用的叶绿素荧光诱导动力学参数与33个小麦品种冻害程度的相关性,筛选出与小麦冻害发生程度呈显著正相关的指标TR_(o)/CS_(m),相关系数达到了0.636,相关性大于前人所用叶绿素荧光指标F_(v)/F_(m)。表明叶绿素荧光参数TR_(o)/CS_(m)可以用来评价小麦的抗寒性,该结果为冬小麦越冬期管理和抗寒育种提供了有力支持。Freezing damage is a common natural disaster in wheat production.At present,the methods for nondestructive evaluation of freezing damage of wheat are still very limited.Rapid chlorophyll fluorescence induction kinetics curve contains abundant information about photosynthesis,which is a common method to study environment stress.By screening suitable chlorophyll fluorescence parameters to evaluate the cold resistance of wheat,it provides technical support for the cold resistance mechanism,overwintering management and cold resistance breeding of wheat.In this study,by investigating the correlation between 34 commonly used chlorophyll fluorescence induction kinetic parameters and frost damage degree of 33 wheat varieties in the year of freezing injury occurrence in overwintering period,TR_(o)/CS_(m) was screened out as an indicator with significant positive correlation with frost damage degree of wheat,and the correlation coefficient reached 0.636,which correlation was better than the previous chlorophyll fluorescence index F_(v)/F_(m).It indicates that the chlorophyll fluorescence parameter TR_(o)/CS_(m) can be used to evaluate the cold resistance of wheat.The findings of this study provided strong support for winter wheat overwintering management and cold resistance breeding.

关 键 词：小麦 低温冻害 抗寒性 叶绿素荧光 

分 类 号：S512.1[农业科学—作物学] S426