白藜芦醇可有效改善铅引起的精原细胞和睾丸间质细胞毒性  

Effectively improvement of resveratrol on the toxicity of spermatogonia and testicular stromal cells caused by lead

作　　者：叶臻[1] 李莉华[1] YE Zhen;LI Lihua(Reproductive Medicine Center,Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430013,China)

机构地区：[1]华中科技大学同济医学院生殖医学中心,湖北武汉430013

出　　处：《中国优生与遗传杂志》2021年第8期1046-1050,共5页Chinese Journal of Birth Health & Heredity

摘　　要：目的本研究的目的是研究白藜芦醇(Res)在间质细胞凋亡过程中的抑制作用及其分子机制。方法通过H-E染色与免疫组织化学分析正常生精患者、生精功能低下患者和SCOS患者及体内模型中NDRG2(N-myc down-stream regulatedgene2)的表达情况,通过末端脱氧核苷酸转移酶末端标记(TUNEL)和免疫荧光分析不同处理后间质细胞的凋亡,采用PCR检测不同处理后NDRG2的表达。结果NDRG2通常只定位于间质细胞的胞质,NDRG2在凋亡刺激后上调并移位到细胞核中,同时NF-κB被乙酸铅(PbAc)激活,进一步增加NDRG2表达,这种作用可被白藜芦醇阻断。结论白藜芦醇可抑制NF-κB介导的NDRG2通路,进而减少睾丸间质细胞凋亡,该发现在睾丸间质细胞凋亡和男性生育研究中有重要作用。Objective The purpose of this study was to study the role of resveratrol in interstitial cell apoptosis and its molecular mechanism.Methods To analyze the expression of NDRG2 in patients with normal spermatogenesis,low spermatogenesis patients and SCOS(sertoli-cell-only syndrome)patients and models in vivo,H-E and immunohistochemistry,terminal deoxynucleotide transfer enzyme end labeling(TUNEL)and immunofluorescence were used to analyze the apoptosis of mesenchymal cells after different treatments,and PCR was used to detect the expression of NDRG2 after different treatments.Results NDRG2 was usually located only in the cytoplasm of stromal cells.NDRG2 was up-regulated and translocated to the nucleus after apoptosis.At the same time,transcription factor NF-κB was activated by PbAc,which further increased the expression of NDRG2,which could be blocked by resveratrol.Conclusion Resveratrol can inhibit NF-κB-mediated NDRG2 pathway,thereby reducing testicular stromal cell apoptosis.This finding may play a role in the area of testicular stromal cell apoptosis and male fertility.

关 键 词：NF-ΚB 间质细胞 细胞凋亡 不孕症 

分 类 号：R285[医药卫生—中药学]