miR-9介导HIF-1α通路对妊娠滋养细胞氧化应激损伤的影响  

Effects of mi R-9-mediated HIF-1αPathway on Oxidative Stress Damage of Gestational Trophoblasts

作　　者：金良怡[1] 尹智华[2] JIN Liangyi;YIN Zhihua(Shenyang Women’s and Children’s Hospital,Shenyang 110011,China;不详)

机构地区：[1]沈阳市妇婴医院,辽宁沈阳110011 [2]中国医科大学

出　　处：《中国医学创新》2022年第2期23-27,共5页Medical Innovation of China

摘　　要：目的:探讨miR-9介导缺氧诱导因子-1α(HIF-1α)通路对妊娠滋养细胞氧化应激损伤的影响。方法:对数生长期的JEG-3细胞随机分为对照组、miR-NC组与miR-9组,miR-NC组与miR-9组转染终浓度为100 nmol/L的miR-NC与miR-9 mimics,对照组转染等体积的磷酸盐缓冲液。采用MTT法检测细胞增殖,Annexin V/FITC检测细胞凋亡,Transwell小室实验检测细胞侵袭,酶联免疫法检测氧化应激损伤指标,蛋白质印迹法检测HIF-1α蛋白表达。结果:转染后24、36 h,miR-9组细胞侵袭指数与细胞增殖指数均明显较对照组与miR-NC组低(P<0.05),细胞凋亡指数均高于对照组与miR-NC组(P<0.05)。转染后24、36 h,miR-9组的SOD含量均高于对照组与miR-NC组(P<0.05),MDA含量、HIF-1α蛋白相对表达水平均低于对照组与miR-NC组(P<0.05)。结论:miR-9在妊娠滋养细胞的高表达能抑制HIF-1α的表达,维持氧化应激处于平衡状态,从而促进细胞凋亡,抑制细胞增殖与侵袭。Objective:To investigate the effects of miR-9-mediated Hypoxia-inducible factor-1α(HIF-1α)pathway on the oxidative stress damage of gestational trophoblasts.Method:JEG-3 cells in logarithmic growth phase were randomly divided into three groups-control group,miR-NC group and miR-9 group,miR-NC group and miR-9 group were transfected with miR-NC and miR-9 mimics at final concentration of 100 nmol/L,the control group were transfected with equal volume of phosphate buffer.MTT method were used to detect cell proliferation,Annexin V/FITC were to detect cell apoptosis,Transwell chamber were to detect cell invasion,enzyme-linked immunoassay were to detect oxidative stress damage indicators,Western blotting were to detect HIF-1αprotein expression.Result:At 24 h and 36 h after transfection,the cell proliferation index and cell invasion index of the miR-9 group were lower than those of the the control group and miR-NC group(P<0.05),and the apoptosis index were higher than those of the the control group and miR-NC group(P<0.05).At 24 h and 36 h after transfection,the SOD content of miR-9 group were higher than those of the control group and miR-NC group(P<0.05),MDA content and relative expression levels of HIF-1αprotein were lower than those of the control group and miR-NC group(P<0.05).Conclusion:The high expression of miR-9 in gestational trophoblasts can inhibit the expression of HIF-1α,maintain oxidative stress in balanced state,promote cell apoptosis,inhibit cell proliferation and invasion.

关 键 词：MIR-9 妊娠滋养细胞 缺氧诱导因子-1Α 氧化应激 细胞凋亡 细胞增殖 

分 类 号：R714[医药卫生—妇产科学]