2015-2019年四川省布鲁氏菌多位点可变数目串联重复序列基因分型研究  

Multiple locus variable-number tandem repeat analysis on Brucella isolated in Sichuan,2015-2019

作　　者：李文博 周汉洪[2] 闫国栋[3] 胡馨月 罗春花 田国忠[6] 杨小蓉[1] 曾林子[1] 廖虹瑜[1] 祁腾[1] 姜海[6] Li Wenbo;Zhou Hanhong;Yan Guodong;Hu Xinyue;Luo Chunhua;Tian Guozhong;Yang Xiaorong;Zeng Linzi;Liao Hongyu;Qi Teng;Jiang Hai(Institute of Microbiological Detection and Analyses,Sichuan Provincial Center for Disease Control and Prevention,Chengdu 610041,Sichuan,China;Dazhou Prefectural Center for Disease Control and Prevention,Dazhou 635000,Sichuan,China;Zigong Prefectural Center for Disease Control and Prevention,Zigong 643000,Sichuan,China;Luzhou Prefectural Center for Disease Control and Prevention,Luzhou 646000,Sichuan,China;Institute of Acute Communicable Disease Prevention and Control,Sichuan Provincial Center for Disease Control and Prevention,Chengdu 610041,Sichuan,China;State Key Laboratory of Infectious Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]四川省疾病预防控制中心微生物检验所,四川成都610041 [2]达州市疾病预防控制中心卫生检验中心,四川达州635000 [3]自贡市疾病预防控制中心,四川自贡643000 [4]泸州市疾病预防控制中心,四川泸州646000 [5]四川省疾病预防控制中心急性传染病预防控制所,四川成都610041 [6]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,感染性疾病协同诊治协同创新中心,北京100026

出　　处：《疾病监测》2021年第12期1281-1285,共5页Disease Surveillance

基　　金：四川省科技计划(No.2020YFS0581)。

摘　　要：目的使用多位点可变数目串联重复序列分析(MLVA)对四川省2015—2019年人间布鲁氏菌病(布病)分离菌株进行分型研究,为当地人间布病防控工作提供参考。方法选用MLVA-16方法对四川省布鲁氏菌株进行分型实验,通过在线数据库对比MLVA-8型别,利用Bio Numerics对MLVA-16位点重复数进行聚类分析。结果四川省布鲁氏菌株被分为29个MLVA-16基因型,遗传相似度在74.6%~100.0%,具有42、43、83共3个MLVA-8型,三者均起源于东地中海群,42型为主要MLVA-8基因型。MLVA-8方法分辨率为0.356,MLVA-16分辨力为0.991,其中Bruce04、Bruce16及Bruce30位点分辨力较高,可采用MLVA-16方法作为本地暴发调查和散发疫情监测手段。结论四川省主要流行株为42型(MLVA-8型别)羊种布鲁氏菌,与全国流行株一致,提示该省布病疫情与北方疫情存在关联。本研究首次将四川省布鲁氏菌进行MLVA分型,利用其构建的数据库将对四川省未来的布病监测及分子溯源工作提供基础数据支持。Objective To understand the genotype of Brucella isolated in Sichuan Province and provide scientific evidence for the prevention and control of brucellosis.Methods A total of 33 Brucella isolates were genotyped by multiple locus variable-number tandem repeat analysis(MLVA)-16.MLVA-8 genotype was queried by online public database,and cluster analysis of MLVA-16 was performed with UPGMA using software Bio Numerics.Results The Brucella isolates were divided into 29 MLVA-16 genotypes with genetic similarity ranging from 74.6%to 100.0%.There were three MLVA-8 genotypes,including 42,43 and 83,all of which originated from the Eastern Mediterranean group,and type 42 was predominant.The Simpson’s index of MLVA-8 was 0.356,while that of MLVA-16 was 0.991.MLVA-16 can be used for local outbreak investigation and sporadic case surveillance.Conclusion Brucella melitensis was the main pathogen causing brucellosis in Sichuan,and type 42(MLVA-8 genotype)was the predominant genotype,consistent with strains isolated in other areas in China,suggesting that there was a correlation in incidence of brucellosis between Sichuan and provinces in northern China.For the first time,Brucella strains isolated in Sichuan were classified by MLVA,and the database constructed by them would provide basic data support for the surveillance and molecular traceability of brucellosis.

关 键 词：布鲁氏菌病 多位点可变数目串联重复序列分析 羊种布鲁氏菌 

分 类 号：R211[医药卫生—中医学] R516.7